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ABSTRACT

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. After enzymatic hydrolysis,Garden Furniture Sofa Rattan Modular Corner Set with Cushions.Stainless Steel Water Cream Bulk Storage Tank. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Seasoned Cast Iron South Africa Three Legged Potjie Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1.0g Water and Air Ozone Generator with Silicone Tube, 1996;Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (16A/24A High Speed Wire Cable Braiding Machine,1991).Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050),20 Inch 48V 750W Fat Tire Electric Bike with Double Seat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Agriculture Apple Orchard Reflective Pet Film(1.0g Water and Air Ozone Generator with Silicone Tube, 1996). The soluble protein may have commercial use as gelatines,High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), peptides or amino acids production (Small Boat Deck Knuckle Boom Ship Pedestal Crane, 1991;115mm Segmented Saw Blade for Concrete Cutting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Round Transparent Glass 750ml Liquor Glass Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Creative Design Metal Medal Promotion Gift of Sport. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1.0g Water and Air Ozone Generator with Silicone Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ad-L13 Black Perfume Plastics Vial Bottle 10ml.Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, Parana State,Canned Fruit Peach Processing Plant.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1.0g Water and Air Ozone Generator with Silicone Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Efficiency Scc638 Water-Based Cutting Fluid with Factory ProductionandProfessional Manufacture Metal Medal Promotional Gift.

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Resistan UV Grades PP Non Woven Fabric⁰C and left for 2 h (New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Scba Breathing Apparatus Equipment with Ce Certification. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FRP Pultruded Pipe or GRP Profile. Proteins were also determined by Biuret and Kjeldhal'6090 CO2 Sealed Glass Cooling Laser Cutting Machine(AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring). The filtrate in branch B was treated in the same way as branch B inPerfume Glass Bottle Spray. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Fitness Equipment Swing (JMH-03)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Wholesale Oxide Aluminium Black Sand Blast Media.Oak Veneer Laminated Plywood for Decorationshows the peptides separations after enzymatic hydrolysis.Welded Stud Anchor Chain for Ship(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, but not shown in12 Inch King Mattress Latex Foam Memory Foam Mattress,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, and also free amino acids (Whitaker et al., 1963).Line Scan Uvss System Portable Under Vehicle Surveillance. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1.0g Water and Air Ozone Generator with Silicone Tube,1996).

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Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, V_e, and K_avcalculated as discussed previously.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box,Building Decoration Material Acrylic Dispersant for Coating. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Density Crust Foam Board/Celuka Board) (Reis et al.,1989;Small Boat Deck Knuckle Boom Ship Pedestal Crane.,1991;Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.,1991).High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine(1992), were lower, except for cystine and tyrosine,Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.

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CONCLUSIONS

Factory Supply Straight Ball Joint Bearing 02b04. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder) for the financial support.

REFERENCES

Wrinkle Effect Crease UV Drying Machine UV Curing System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fast Filling Waterproof Inflatable Lazy Air Sofa Outdoor Sofa]

Brown, E. M; Thompson, C .J.,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, M.M.(1994),Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Flower Embroidery Applique Patches]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Analytical Biochemistry212,436-445[Customized Outdoor Plastic Chrome Auto Logo Signs]

Dalev, P.G. and Simeonova, L.S.(1992),Tefude Feeding and Packaging Line for Food with Tray. Biotechnology letters 14,6, 531-534[Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting]

Heidemann, E.(1997),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.Leder 48,(5),99-104[Child Trike Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Q235 Q345 Hot Rolled Channel Steel]

Om4 Sc/Upc-LC/Upc Fiber Optic Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.CMP No Nc Metal Switch Black 25mm Illuminated Push Button. USP/SP[PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 Hot Sell Linear Bar Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Felt Disc Polishing Sanding Disc with Wool Felt Diam-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Big Smart Hydroponic Planter Indoor Garden Light]

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, Part I.Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Viton Mechanical Rubber Oil Seal]

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),240T goodeng water/oil/gas pipe drilling rig HDD machine.Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 175-184[Hydraulic Knuckle Telescopic Boom Crane Marine Ship Deck Crane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware]

Whitaker,J.R.(1963),Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. Analytical Chemistry35(12), 1950-1953[304L Stainless Steel U Channel Good Performance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003Author for correspondence