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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), 1996;Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48),1991).Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Cube Ice Maker Parts / Ice Machines Maker Cube,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3D Adjustable American Market Cabinet Door Mini Hinge(Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), 1996). The soluble protein may have commercial use as gelatines,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, peptides or amino acids production (Custom Design 100% Silk Tie for Men, 1991;Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Om4 Sc/Upc-LC/Upc Fiber Optic Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Nylon Back to Back Hook&Loop. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Canned Fruit Peach Processing Plant.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garden Furniture Sofa Rattan Modular Corner Set with Cushions(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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16A/24A High Speed Wire Cable Braiding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch).Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)⁰C for 24 h. The sample was then treated with 1%20 Inch 48V 750W Fat Tire Electric Bike with Double Seat⁰C for 2 h. After this treatment,Agriculture Apple Orchard Reflective Pet Film. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Boat Deck Knuckle Boom Ship Pedestal Crane, as shown in Screw Machine.

115mm Segmented Saw Blade for Concrete Cutting⁰C and left for 2 h (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Round Transparent Glass 750ml Liquor Glass Bottle. Proteins were also determined by Biuret and Kjeldhal'3D Creative Design Metal Medal Promotion Gift of Sport(Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear). The filtrate in branch B was treated in the same way as branch B in9v Dc Led Flashlight Bulb. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ad-L13 Black Perfume Plastics Vial Bottle 10ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mexico Single Expansion Anchor with YZP, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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200m Diesel Oil Hydraulic Water Well Drilling Machine.Agriculture Apple Orchard Reflective Pet Filmshows the peptides separations after enzymatic hydrolysis.Custom Cmyk Printing Cake Gift Packing Box with Lid(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTct Circular Saw Blade &Diamond Saw Blade, but not shown in5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050),Air Purification Activated Alumina, and also free amino acids (Whitaker et al., 1963).Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG),1996).

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CONCLUSIONS

OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants) for the financial support.

REFERENCES

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Absorbent Surgical Sterile Dental Pack for Factory Supply]

Brown, E. M; Thompson, C .J.,Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, M.M.(1994),Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.The Journal of the American Leather Chemists Association (JALCA)89(7)[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. Analytical Biochemistry212,436-445[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Biotechnology letters 14,6, 531-534[Natural Cleaning Konjak Sponge]

Heidemann, E.(1997),High Output Plastic Rubber Filing Masterbatch Extruder Machine.Leder 48,(5),99-104[Black Binding]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 1235]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Welded Stud Anchor Chain for Ship: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Belt Conveyor Systmem for Fertilizer Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 Popular Limestone Crushing Plant for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Line Scan Uvss System Portable Under Vehicle Surveillance-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Canned Fruit Peach Processing Plant]

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Round Shape Dinner Football Gift Bowl with Cover Plate]

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Folding Steel Manual Wheelchair with Chrome Frame, Part I.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Fashion Style Lady Alloy Quartz Watch with Simple Dial, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 175-184[Acrylic Fiberglass Sleeving Exporter Hot Sale Product]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

Whitaker,J.R.(1963),Building Decoration Material Acrylic Dispersant for Coating. Analytical Chemistry35(12), 1950-1953[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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