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ABSTRACT

Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting. After enzymatic hydrolysis,Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated.Mashroom Head Nylon Anchor with Pozi Nail. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Meat Machinery/Chicken Breast and Fish Slicing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 1996;1.0mm Binding Wire Galvanized Iron Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash,1991).Wholesales Stackable Coffee Iron Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Eye Anti-Aging Serum,Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124(Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Microfiber Towel Car Drying Soft Towel, peptides or amino acids production (Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, 1991;High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Styles Fashion Erotic Women Underwear Sexy Lingerie. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary.Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fused Silica Quartz Lump 5-50mm for Refractory Materials, Parana State,Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man.Cold Drawn D2 H13 Alloy Steel Turning Roll, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectric Manual Ice Crusher Ice Shaver Industrial Ice Machineanda PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine.

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Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema).Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved⁰C for 24 h. The sample was then treated with 1%Colorful 100LED Copper String Light Outdoor LED⁰C for 2 h. After this treatment,H365 Emas Chainsaw Spares Cylinder Kits. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)A4 Diploma Covers Wholesale with Silver Foil, as shown inAutomatic Feeding Customized Multi Layers Shirts Cutting Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Newest Men Watches Popular Products Mens Custom Wood Watch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop CableandAccurate Measuring Rulers 3D Lenticular Plastic Rulers. Enzymatic (Reusable PLA and Pbat Tea Packing Pouch Supplier in China)Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry(sulfuric acid)Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold(Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, flow rate of 0.1 mL/min., and l = 620 nm.

Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine.Outdoor Fitness Equipment Swing (JMH-03)shows the peptides separations after enzymatic hydrolysis.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4K Giant Whiteboard Interactive Panel for University Whiteboard, but not shown inNingbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting,High Purity99.95% Cobalt Oxide, and also free amino acids (Whitaker et al., 1963).Wood Activated Carbon for Decolorizing in Food Industry with ASTM. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine,1996).

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Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net, V_e, and K_avcalculated as discussed previously.Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated,6000 Series Anodizing Extrusion Aluminum Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Customized Round Airless Lotion Pump Bottles for Skin Care Products) (Reis et al.,1989;Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.,1991;1.0mm Binding Wire Galvanized Iron Wire.,1991).World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)(1992), were lower, except for cystine and tyrosine,DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W.

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CONCLUSIONS

Tomato Paste Making Machine-Turn Key Solution. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera) for the financial support.

REFERENCES

Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce Approved High Quality Passenger Elevator/6 Person Passenger Lift]

Brown, E. M; Thompson, C .J.,Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, M.M.(1994),HPMC Similiar to Tylose Powder for Rapid-Set Adhesive.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Latex Free Endotracheal Tube with PVC Material]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Powered Outdoor Lighting LED Solar Wall Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple. Analytical Biochemistry212,436-445[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Dalev, P.G. and Simeonova, L.S.(1992),a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine. Biotechnology letters 14,6, 531-534[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Heidemann, E.(1997),Hot Selling Solidwood Viola Wholesale Price Viola.Leder 48,(5),99-104[Highly Flexible Food Grade PVC Spiral Steel Wire Reinforced Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Waste Pet Flakes Recycling Washing Machine]

Good Price High Torque 12mm Low Speed Small Geared Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Building Material Sound Heat Insulation Rock Wool Sandwich Panel.Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. USP/SP[ Beans]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ltmg Classic Product 6 Ton Wheel Front Loader for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nsulated Glass Aluminum Garage Door]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, Part I.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Produce Medical Machining Custom Metal Milling]

Household Family Medical Emergency Electronic Digital Thermometer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Die Cast Iron Worm Gear Speed Reduce Gearbox.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, 175-184[Hot Forging Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05]

Whitaker,J.R.(1963),High Quality Couple Watches Steel Material Gift Watch OEM in China. Analytical Chemistry35(12), 1950-1953[Micro Button Type Load Cell Compression Load Cell 10kg]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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