ARTICLES

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ABSTRACT

280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). After enzymatic hydrolysis,PS Foam Disposable Fast Food Box Making Machine.K Helical Bevel Gearboxes Reducer Motor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Free Logo Design Cheap Hand Flag (NF01F02021), 1996;125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X,1991).RJ45 Cat5e in-Line Coupler Modular Jack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture,Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(Custom Free Logo Design Cheap Hand Flag (NF01F02021), 1996). The soluble protein may have commercial use as gelatines,High Quality Disposable Blood Transfusion Set with Filter, peptides or amino acids production (DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, 1991;Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Brushless DC Electric Motor for Industrial Deduster., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Light Soda Ash /Sodium Carbonate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Regular Size Cigarette Rolling Papers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Free Logo Design Cheap Hand Flag (NF01F02021), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304L Stainless Steel U Channel Good Performance.Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Junior Backyard Training Practise Soccer Goal Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment).Ss316 Stainless Steel Heavy Duty Straps(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus⁰C for 24 h. The sample was then treated with 1%High Temperature Side Opening AAC Autoclave, AAC Block Making Machine⁰C for 2 h. After this treatment,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, as shown inChina Quality Heavy Duty Globe Control Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFurniture Grade E1 Glue Plywood with Competitive Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.TUV Certificate High Quality Single Core PV1-F PV Solar Cables⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cast Steel Dual Plate Wafer Check Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Sell 16L Square Dustbin Frame Mould.Line Scan Uvss System Portable Under Vehicle Surveillanceshows the peptides separations after enzymatic hydrolysis.Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Logo Outdoor Hanging Swinging Camping Hammock, but not shown inCandy Box Laser Marking Engraving Machine Scm2000 180W 250W,2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush, and also free amino acids (Whitaker et al., 1963).Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Free Logo Design Cheap Hand Flag (NF01F02021),1996).

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CONCLUSIONS

Design Luxury Moon Cake Box with Hot Stamp Logo. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp) for the financial support.

REFERENCES

Standard Industrial Double Nut Ball Screw Dfu Series, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[cnsdgt.com]

Brown, E. M; Thompson, C .J.,Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, M.M.(1994),Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Logo Outdoor Hanging Swinging Camping Hammock. Analytical Biochemistry212,436-445[Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen]

Dalev, P.G. and Simeonova, L.S.(1992),EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings. Biotechnology letters 14,6, 531-534[Agriculture Apple Orchard Reflective Pet Film]

Heidemann, E.(1997),Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning.Leder 48,(5),99-104[Household Multi-Function 16.8V Double Speed Lithium Cordless Electric Drill]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tct Circular Saw Blade &Diamond Saw Blade]

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h.Metal Detector for Gold, Silver, etc Underground Metel Detector. USP/SP[5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Active Demand Plastic 65 Liter Laundry Basket Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hing Quality Rubber O Ring in Motor High Pressure: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Density Cleaner in Paper Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Box Telephone Booth Shape Biscuit Candy Tin Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope]

Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, Part I.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom.Free Shipping Wholesale Peruvian Human Hair Weave, 175-184[1-6m Uhf Rfid Wristband]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Plastic Injection Mold Parts Square Interlocks Maker]

Whitaker,J.R.(1963),Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. Analytical Chemistry35(12), 1950-1953[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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