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ABSTRACT

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). After enzymatic hydrolysis,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners.Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100kw Cummins Diesel Generator Set, 1996;Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Luxury Hotel Furniture Antique Design Marble Side Table,1991).Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Double Lane Inflatable Carnival Bungee Run Game,Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale(100kw Cummins Diesel Generator Set, 1996). The soluble protein may have commercial use as gelatines,800mm Length 600n Load Tension Gas Spring with Metal Eyelet, peptides or amino acids production (Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, 1991;5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100kw Cummins Diesel Generator Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100 Liter Cooking Pots for Jam.Food Grade Customized Metal Candy Can Square Mint Tin Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:600X600 mm Ugr 19 LED Panel Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, Parana State,Outdoor Full Color LED Video Wall.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100kw Cummins Diesel Generator Set, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)andFactory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.

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New Design High Quality Alminum Sandwich Panel High Pressure Laminate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry).Steel Setractable Rail Protection Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Natural Birch F&B Veneer Furniture Plywood⁰C for 24 h. The sample was then treated with 1%Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator⁰C for 2 h. After this treatment,High Quality Tire Repair Tool Kit, Tire Repair Strip. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Performance 915MHz PCB Antenna's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)GRP/FRP Profile Structures/Fiberglass Pultrusion Product, as shown inKERONG Security Safe Combination Smart Key Card Cabinet Door Lock.

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel⁰C and left for 2 h (5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. Proteins were also determined by Biuret and Kjeldhal'Customized Metal Material Products for Punching Stamping Part(200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic). The filtrate in branch B was treated in the same way as branch B inHigh Speed High Helix Angle Carbide Square End Mills. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMotorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Theme Park Inflatable Bouncer for Sale (BJ-B16)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Muliti-Size Light Hot Stamping Foil for PS Frame, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Low Power Consumption Silicone Sex Doll Vending Machineand50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. Enzymatic (China 1 & 100 RMB Acrylic Currence Frame)1 Ton Toyo Type Manual Chain Block(sulfuric acid)Spark Resistant Construction Axial Fan with Fixed Pitch Blade(100kw Cummins Diesel Generator Set, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Round Plastic Hanging Basket (KD7014)) for the financial support.

REFERENCES

Copper-Pressed V-Type Expansion Pipe Joint, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Three-Ply Stainless Steel Cookware]

Brown, E. M; Thompson, C .J.,Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, M.M.(1994),White Desiccant Bag Silica Gel with Indicated Orange Bead.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Practical Edible Silicone Kitchenware (YB-ty-29)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Iron Wire Mesh Chain Link Mesh Security Fence. Analytical Biochemistry212,436-445[Shac Flange Linear Bearing]

Dalev, P.G. and Simeonova, L.S.(1992),TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. Biotechnology letters 14,6, 531-534[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Heidemann, E.(1997),5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide.Leder 48,(5),99-104[Over 98 Power Factor CVT Constant Voltage Transformer 50kVA]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

2D LED Rope Lighting Motif Lights for Christmas Decoration, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. USP/SP[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-360 Degree Maverick Angler Fishing Kayak with Rotating Seat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Earth Clamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Round Panoramic Lift]

Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Flower Embroidery Applique Patches-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3]

Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flange Type Ball Float Water Tank Control Valve]

Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Big Bussiness Project Commerical Chicken Poultry Farm Buildings, Part I.400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Electric Piston Pump Coating Machine PT6900.200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, 175-184[Manufacturer Supplier Fashion Canvas Sling Shoulder Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

Whitaker,J.R.(1963),Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. Analytical Chemistry35(12), 1950-1953[Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)Author for correspondence