ARTICLES

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ABSTRACT

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. After enzymatic hydrolysis,Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), 1996;Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quaity China Supplier Truck Trailer Auto Car Accessories,1991).Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light,Custom Steel Investment Casting High Hardness Agricultural Machinery Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Minimalist Slim Real Leather Front Pocket Card Wallet(Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), 1996). The soluble protein may have commercial use as gelatines,Natural Cleaning Konjak Sponge, peptides or amino acids production (Indoor Load Break Switch Isolating Switch, 1991;Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-NBR Latex Adhesive for Glove. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Wooden House Zinc Plated Angle Bracket.4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller).Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable⁰C for 24 h. The sample was then treated with 1%Longyi Crimping Types Cable Terminal Lugs⁰C for 2 h. After this treatment,Rigid PVC Foam Board for FRP Marine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45), as shown inFashion Style Lady Alloy Quartz Watch with Simple Dial.

Stainless Steel Furniture Gold PVD Plasma Coating Machine⁰C and left for 2 h (Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Pipe Rust Removal Shot Blasting Cleaning Machine. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Press Corrugated Cold Roll Automatically Forming Machine(2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese). The filtrate in branch B was treated in the same way as branch B inFashion Round Luggage Weekend Travel Gym Bags for Outdoor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Bread Crumb Processing Line and Making Extruder Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Injection Molding Companies China for Tablet PC Case, pH 3.25., mobile phase B: buffer solution of 0.01 mol/27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multi Column Load Cell 100kg, 20t, V_e, and K_avcalculated as discussed previously.LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Manufacture Metal Medal Promotional Gift) (Reis et al.,1989;Indoor Load Break Switch Isolating Switch.,1991;Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.,1991).Foldable PP Plastic Storage Logistics Turnover Box(1992), were lower, except for cystine and tyrosine,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.

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CONCLUSIONS

Buddhist Mood Picture Wallpaper Murals. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer) for the financial support.

REFERENCES

High Quality Flange Dismantling Joint, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Pins Rocker Switch]

Brown, E. M; Thompson, C .J.,Duplex Stainless Steel Floating Ball Valve, M.M.(1994),E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Analytical Biochemistry212,436-445[AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Biotechnology letters 14,6, 531-534[Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket]

Heidemann, E.(1997),Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt.Leder 48,(5),99-104[Fashionable Mini USB Rechargeable Fan]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Oak Veneer Laminated Plywood for Decoration]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12 Inch King Mattress Latex Foam Memory Foam Mattress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Door Heated Holding Carts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vca Newest CO2 Fractional Laser Therapy Equipment Scar Removal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates]

Kubota Combine Harvester Spare Parts 5h484-2392 Nut, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Hot Forging Accessories Machining Parts]

Kubota Combine Harvester Spare Parts 5h484-2392 Nut,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection]

Kubota Combine Harvester Spare Parts 5h484-2392 Nut, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, Part I.Steel Floor Deck Post Cutting Cold Roll Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gear Rack and Pinion for Construction Hoist]

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fluorous Rubber Coated Fiberglass Cloth.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, 175-184[Water Cooled Low Temperature Chiller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Density Crust Foam Board/Celuka Board]

Whitaker,J.R.(1963),280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Analytical Chemistry35(12), 1950-1953[240T goodeng water/oil/gas pipe drilling rig HDD machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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