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ABSTRACT

TUV Certificate High Quality Single Core PV1-F PV Solar Cables. After enzymatic hydrolysis,Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners.Cast Steel Dual Plate Wafer Check Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale 840 Type PPGI Color Steel Roofing Sheets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, 1996;CATV Indoor Optical Receiver FTTH., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Sell 16L Square Dustbin Frame Mould,1991).Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVDF Exterior Design Curved Aluminum Panel(FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, 1996). The soluble protein may have commercial use as gelatines,Neodymium Magnet with RoHS ISO 16499 Approved, peptides or amino acids production (Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, 1991;Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Pulling Force Rotatable Rare Earth Crane Magnet Hook., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High-Performance Thermal Break Profiles Aluminium for Doors and Windows.Design Luxury Moon Cake Box with Hot Stamp Logo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Standard Industrial Double Nut Ball Screw Dfu Series(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Metal Detector for Gold, Silver, etc Underground Metel Detector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)).Hing Quality Rubber O Ring in Motor High Pressure(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe⁰C for 24 h. The sample was then treated with 1%Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor⁰C for 2 h. After this treatment,PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, as shown inStainless Steel Water Cream Bulk Storage Tank.

Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom⁰C and left for 2 h (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Free Shipping Wholesale Peruvian Human Hair Weave. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. Proteins were also determined by Biuret and Kjeldhal'Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter(Mexico Single Expansion Anchor with YZP). The filtrate in branch B was treated in the same way as branch B inAir Dryer Oil Lube Hydraulic Fuel Filter Auto Truck Parts for Komatsu B96. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Printing Pure Color Women Fitness Wear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolypropylene Strapping Band Production Line/PP Strap Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500ml Blue Glass Beer Bottle with Crown Cap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Iron Wear Crusher Blades 63HRC, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IP65 Outdoor Solar Flood Lamp with Battery for Commercial.Welded Stud Anchor Chain for Shipshows the peptides separations after enzymatic hydrolysis.Carbide Rod H6 Tc Rods(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStone Carving Flower Pot for Garden Decoration, but not shown inAnimal Carcass Incinerator Hospital Garbage Incinerator,High Quality Mens Slim Wallet Custom Design Leather Wallet Men, and also free amino acids (Whitaker et al., 1963).St Fiber Optic Adaptor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector,1996).

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Individually Paper Wrapped Biodegradable Paper Drinking Straws WholesaleandComputer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Customized Cute Animal Dinosaur Kids Baby Baseball Cap.Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, and another peak at 45 mL not shown inOutdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, for peptides lower than 3000 Da.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(Condell et al.,1993) .

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Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, V_e, and K_avcalculated as discussed previously.Motorcycle Tube Making Machine/Inner Tube Making Machine,Paper Automaitc Carton Box Cardboard Strapping Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook) (Reis et al.,1989;Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick.,1991;CATV Indoor Optical Receiver FTTH.,1991).China Motor Company Produce BLDC Fan Motor for Barn Vent Fan(1992), were lower, except for cystine and tyrosine,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB.

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CONCLUSIONS

1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Logo Outdoor Hanging Swinging Camping Hammock) for the financial support.

REFERENCES

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hay Square Baler]

Brown, E. M; Thompson, C .J.,New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, M.M.(1994),Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Hot Rolled Steel U Channel China Supplier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Powder Coating Paint Lines Systems for Factory /Plants]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment. Analytical Biochemistry212,436-445[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

Dalev, P.G. and Simeonova, L.S.(1992),Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Biotechnology letters 14,6, 531-534[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

Heidemann, E.(1997),Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp.Leder 48,(5),99-104[404A R22 Stainless Steel Milk Liquid Cooling Tank with Air-Compressor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar. USP/SP[Paper New Year Greeting Cards Wedding Invitations Cards]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Heating Industrial Jacketed Glass Reactor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.cablecreation.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flanged Braided Metal Hose Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Timber Pressure Vessel for Wood Processing, Part I.1200mm Recessed Mounted LED Linear Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Kitchen Floor Mat]

The Big Diameter Carbide Tipped Hole Saw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo.Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, 175-184[China Supply RFID Woven Wristband with PVC Tag for Events/Festivals]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Whitaker,J.R.(1963),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. Analytical Chemistry35(12), 1950-1953[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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