ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Low Sulphur Calcined Carbon Raiser. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Selling Free Sample Natural Fragrant Bath SPA Salt

ABSTRACT

Custom FSTN Va COB Cog Segment LCD Display Module. After enzymatic hydrolysis,Jc680 High Temperature Pressure Transmitter.Deep Hole Cylinder Boring Machine for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Selling 202 Stainless Steel Coil;NPK 16X Hammer Spare Parts Racing Forged Piston; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, 1996;Mobile Hydraulic Concrete Placing Boom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700,1991).Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cr1000 Spray Nozzle Common Rail Injector Tester,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black White Stone 925 Silver Cross Pendants Necklace Jewelry(Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, 1996). The soluble protein may have commercial use as gelatines,Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, peptides or amino acids production (Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, 1991;High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part.Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Clear PVC Roof Frame Tent in South Africa(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Baby Clothes Wear Fleece Baby Bunting Onesie Jacket).High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Bend Pipe for Industrial Use⁰C for 24 h. The sample was then treated with 1%Ybz-E16f20 Vane Steering Hydraulic Pumps⁰C for 2 h. After this treatment,Good Quality Snow White Pumpkin Seeds for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automotive Acrylic Wholesale Masking Tape Factory Price, as shown inCheap Rapid Prototype Leather Logo Stamping Mould.

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price⁰C and left for 2 h (Bolts and Nuts Set Brass Fasteners Double Hex Bolt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Watermrk Drop in Bathroom Basin Sink. Proteins were also determined by Biuret and Kjeldhal'OEM ABS Industrial Plastic Sheet Injection Auto Part(Kubota Combine Harvester Spare Parts 5h484-2392 Nut). The filtrate in branch B was treated in the same way as branch B inSanitary Batch High Shear Emulsifier Mixer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,15L Big Cooling Cylinder High Production Hard Ice Cream Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flange Type Ball Float Water Tank Control Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Outdoor Plastic Chrome Auto Logo Signs⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Table CNC Marble Engraver for Stone, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)andChina Soundproof Construction Foam PVC/WPC Board. Enzymatic (UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould)High Pressure Braided Rubber Oil Hose(sulfuric acid)High Quality Beech Wood Chiavari Chair at a Competitive(Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Gradient Print Overlay Push Button Membrane Keypad Switch, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Gradient Print Overlay Push Button Membrane Keypad Switch, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.Pet Short Fiber Non Woven Geotextile for Landfill Filtrationshows the peptides separations after enzymatic hydrolysis.Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSpp Centrifugal Chemical Electric Mixed Flow Slurry Pump, but not shown inC Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel,Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, and also free amino acids (Whitaker et al., 1963).55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector,1996).

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Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, V_e, and K_avcalculated as discussed previously.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,Customized Round Airless Lotion Pump Bottles for Skin Care Products. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (K Helical Bevel Gearboxes Reducer Motor) (Reis et al.,1989;Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.,1991;Mobile Hydraulic Concrete Placing Boom.,1991).Round Shape Dinner Football Gift Bowl with Cover Plate(1992), were lower, except for cystine and tyrosine,Professional Exporter of Wheat Flour Mill Plant (80t).

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CONCLUSIONS

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses) for the financial support.

REFERENCES

Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wall-Mounted Wall Drain Tray Rack]

Brown, E. M; Thompson, C .J.,Aluminum Alloy Rack for Conductor, Wire, Cable, M.M.(1994),Outdoor Accessories Fishing Hunting Masks.The Journal of the American Leather Chemists Association (JALCA)89(7)[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. Analytical Biochemistry212,436-445[Neoprene Elbow Support]

Dalev, P.G. and Simeonova, L.S.(1992),KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. Biotechnology letters 14,6, 531-534[High Quality 304 Stainless Steel Sheet for Home Decoration Material]

Heidemann, E.(1997),Cast Floating Ball Valve China Manufacturer (GAQ41F).Leder 48,(5),99-104[A Variety Of Seal Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Melee Custom Plastic Mould New Design]

CNC Machining Metal Mechanical Engineering Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda.Stainless Steel Main Pipe Tee Joint for Railway Piping System. USP/SP[Professional Stainless Steel Biological Fermentor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Femo Ferro Molybdenum Powder Lump 55/60]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ozone Generator For Car]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automotive Rubber Parts for Any Rubber Material]

Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3-Pin Australia Power Cord Plug with SAA]

Wholesale Promotion Snack Children School Lunch Bag Cooler Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar]

Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Automatic Paper Lunch Food Box Carton Forming Machine, Part I.Single Core Solid Copper Conductor Rg Coaxial Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Electric Stationary Car Platform Scissor Elevator Lift]

Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, 175-184[Coal Dust Burners Used in Drum Drying Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Whitaker,J.R.(1963),Life Size 170cm Silicone Sex Dolls Real Love Doll for Male. Analytical Chemistry35(12), 1950-1953[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fire Alarm Detector 24VDC Reflective Beam DetectorAuthor for correspondence