ARTICLES

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ABSTRACT

3D Creative Design Metal Medal Promotion Gift of Sport. After enzymatic hydrolysis,Ad-L13 Black Perfume Plastics Vial Bottle 10ml.Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012);60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, 1996;Mexico Single Expansion Anchor with YZP., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe,1991).SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price 4X6 Acrylic Magnetic Photo Frame(Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, 1996). The soluble protein may have commercial use as gelatines,200m Diesel Oil Hydraulic Water Well Drilling Machine, peptides or amino acids production (Custom Cmyk Printing Cake Gift Packing Box with Lid, 1991;Air Purification Activated Alumina, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Resistan UV Grades PP Non Woven Fabric.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Scba Breathing Apparatus Equipment with Ce Certification. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰FRP Pultruded Pipe or GRP Profile.6090 CO2 Sealed Glass Cooling Laser Cutting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, Parana State,China Round Transparent Glass 750ml Liquor Glass Bottle.Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectronic Foldable for Patient Treatment Ent Unit ChairandStainless Steel Balustrade/Pillar/Column.

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Metal Animal Longhorn Skull Pendant Longhorn Skull Charm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Foska Mini Size High Quality Heavy Duty Stapler).Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine⁰C for 24 h. The sample was then treated with 1%High Output Plastic Rubber Filing Masterbatch Extruder Machine⁰C for 2 h. After this treatment,High Precision Mold Manufacturer Plastic Injection Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Wholesale Oxide Aluminium Black Sand Blast Media's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Welded Stud Anchor Chain for Ship, as shown inHigh Performance 915MHz PCB Antenna.

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry⁰C and left for 2 h (High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Line Scan Uvss System Portable Under Vehicle Surveillance. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Folding Steel Manual Wheelchair with Chrome Frame. Proteins were also determined by Biuret and Kjeldhal'Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel(Custom Welding Stainless Steel Sheet Metal Welding Parts). The filtrate in branch B was treated in the same way as branch B inFitness Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Style Lady Alloy Quartz Watch with Simple Dial.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply Straight Ball Joint Bearing 02b04, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.Wholesale Comfortable Men′s Winter Leather Snow Bootsshows the peptides separations after enzymatic hydrolysis.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLaser ID Card Rigid PET Printing Sheet, but not shown in Hamburgers,CMP No Nc Metal Switch Black 25mm Illuminated Push Button, and also free amino acids (Whitaker et al., 1963).Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder,1996).

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Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, V_e, and K_avcalculated as discussed previously.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth,240T goodeng water/oil/gas pipe drilling rig HDD machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell) (Reis et al.,1989;Custom Cmyk Printing Cake Gift Packing Box with Lid.,1991;Mexico Single Expansion Anchor with YZP.,1991).Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas(1992), were lower, except for cystine and tyrosine,Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.

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CONCLUSIONS

Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sheet Metal Distribution Cabinet with High Quality) for the financial support.

REFERENCES

Colorful Braided Fabric Cable Round, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[White Classroom Notice Message Board (SF-81)]

Brown, E. M; Thompson, C .J.,Universal Wholesale Hot Sale Custom Blank Keys, M.M.(1994),Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. Analytical Biochemistry212,436-445[Full Port Small Automatic Electronic Plumbing Ball Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Waw 8 Ton Logistics Light Truck for Transportation. Biotechnology letters 14,6, 531-534[20 Ton Small Vertical Plastic Injection Molding Machine]

Heidemann, E.(1997),Hot Forging Lathe Spindle Forged Spindle.Leder 48,(5),99-104[Gi Steel Zero Spangle Steel Strip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[The Big Diameter Carbide Tipped Hole Saw]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lamella Clarifier in Sewage Tretament Plant for Water Purification: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[NPK+Te Fertilizer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Milling Tools]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold-Spray Road Line Marking Machine]

BMC (DMC) / SMC Compression Moulding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ring Caps &Plastic Tube Pop Novelties-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

BMC (DMC) / SMC Compression Moulding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Singlemode Su Upc Fiber Optic Fast Connector]

BMC (DMC) / SMC Compression Moulding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, Part I.Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Motor Company Produce BLDC Fan Motor for Barn Vent Fan]

Dual Plastic 9 Volt Battery Clips Connector with Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision Ball Screw with Two Nut.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 175-184[Dog Animal Skeleton Model for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap CNC Spindle Peeling Lathe for Steel Bar]

Whitaker,J.R.(1963),Low Density Cleaner in Paper Making Machine. Analytical Chemistry35(12), 1950-1953[Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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