ARTICLES

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ABSTRACT

Electrical Bike Waterproof Power Connector. After enzymatic hydrolysis,Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.Painted G80 Cargo Lashing Chain with Hooks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Powerful Charger with Dual Pd Charger and Type-C Charger, 1996;Flexible Vj Rigid Shaft Coupling for Pipe Flange., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting,1991).Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers,Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price(Portable Powerful Charger with Dual Pd Charger and Type-C Charger, 1996). The soluble protein may have commercial use as gelatines,15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, peptides or amino acids production (High Speed Steel Mill Roll with High Quality, 1991;3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2017 China Hot Sale Water Screen Movie Fountain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Powerful Charger with Dual Pd Charger and Type-C Charger, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip.Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse -LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W).Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cross Hex Flange Screw⁰C for 24 h. The sample was then treated with 1%Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings⁰C for 2 h. After this treatment,Full Color Indoor Outdoor LED Curtain TV Display. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Plastic Injection Mold Parts Square Interlocks Maker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Outdoor Solar Lawn Garden Lighting for Wall, as shown inFactory Supply Straight Ball Joint Bearing 02b04.

3-Pin Australia Power Cord Plug with SAA⁰C and left for 2 h (Customized Cute Animal Dinosaur Kids Baby Baseball Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sludge Oil Separation Centrifuge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8011 Aluminum Foil for Cap Used. Proteins were also determined by Biuret and Kjeldhal'Pepper Peanut Sauce Colliod Miller Grinding Machine(High-Tower Compound NPK Fertilizer 15-15-15). The filtrate in branch B was treated in the same way as branch B inm.rewodacn.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gasoline Rototiller, Cultivator, Tiller Model Gtm-6.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12.5mva Transformer 12500kVA 33kv 35kv Power Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectronic Foldable for Patient Treatment Ent Unit Chair(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Shaft Paper Cardboard Waste Plastic Shredder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).Precision Ball Screw with Two Nutshows the peptides separations after enzymatic hydrolysis.Animal Carcass Incinerator Hospital Garbage Incinerator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWood Fireplace Double Skin Chimney Flue Pipes, but not shown in12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch,Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, and also free amino acids (Whitaker et al., 1963).High Density Crust Foam Board/Celuka Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Portable Powerful Charger with Dual Pd Charger and Type-C Charger,1996).

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CONCLUSIONS

Factory Wholesale Melamine Garment Shop Furniture Design for Store. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Delicate Handmade Round Natural Stone Chain Necklace) for the financial support.

REFERENCES

Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

Brown, E. M; Thompson, C .J.,Wholesale Comfortable Men′s Winter Leather Snow Boots, M.M.(1994),Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Density Cleaner in Paper Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gcc Plant Ball Mill and Air Classifier Production Line. Analytical Biochemistry212,436-445[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes. Biotechnology letters 14,6, 531-534[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

Heidemann, E.(1997),Outdoor Playground Plastic Slide with Swing and Basketry.Leder 48,(5),99-104[300Ton Hydraulic Welding Rotators]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

Machinery Parts Aeroplane Engine CNC Milling, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat.Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components. USP/SP[High Quality Plastic Injection Mold Parts Square Interlocks Maker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Belt Conveyor Systmem for Fertilizer Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvc Plastic Roller Shutter Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

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Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Natural Cleaning Konjak Sponge]

Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.8ml Clear Sample Vials with Label and PP Caps, Part I.Factory Small Coil Compression Spring in High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel.Plastic Material Handling Dolly Platform Hand Trolley Truck, 175-184[Singlemode Sc/APC Shutter Fiber Optical Adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[9~12 Inch Mechanical Backing Plate]

Whitaker,J.R.(1963),Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. Analytical Chemistry35(12), 1950-1953[ 1235]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Belt Conveyor Systmem for Fertilizer Production LineAuthor for correspondence