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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Shac Flange Linear Bearing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price

ABSTRACT

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. After enzymatic hydrolysis,CNC Router for Engraving Cutting Carving Drilling Milling.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa;Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Hot Rolled Steel U Channel China Supplier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1996;Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck,1991).500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable,Anti-Pollution Modified Acrylic Solid Surface for Countertops,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1996). The soluble protein may have commercial use as gelatines,Corrugated Carton Box Fully Automatic Folding Gluing Machine, peptides or amino acids production (800lb 1/2 A105 Thread Forged Steel Globe Valve, 1991;Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Metal Mechanical Engineering Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Sulphur Calcined Carbon Raiser. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Selling Free Sample Natural Fragrant Bath SPA Salt.Custom FSTN Va COB Cog Segment LCD Display Module-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jc680 High Temperature Pressure Transmitter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3).Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cr1000 Spray Nozzle Common Rail Injector Tester⁰C for 24 h. The sample was then treated with 1%Industrial Cast Steel Flanged One Way Swing Check Valve Nrv⁰C for 2 h. After this treatment,Black White Stone 925 Silver Cross Pendants Necklace Jewelry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, as shown inFlange Type Ball Float Water Tank Control Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGarvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home WipesandFactory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. Enzymatic (5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle)Clear PVC Roof Frame Tent in South Africa(sulfuric acid)High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)(M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Bend Pipe for Industrial Use, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Bend Pipe for Industrial Use, flow rate of 0.1 mL/min., and l = 620 nm.

Ybz-E16f20 Vane Steering Hydraulic Pumps.Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)shows the peptides separations after enzymatic hydrolysis.Good Quality Snow White Pumpkin Seeds for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, but not shown inCustomized Double Acting Hydraulic Cylinder for Excavator,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, and also free amino acids (Whitaker et al., 1963).Automotive Acrylic Wholesale Masking Tape Factory Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (M Type Metallic Yarn Lurex Yarn Embroidery Yarn,1996).

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Safety Full Automatic Electric Opening AAC Autoclave for AAC PlantandGood Price Channel Steel Used for Machineryshow the gel chromatographic separation of soluble protein with Sephadex G-Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price.Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, and another peak at 45 mL not shown inCNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, for peptides lower than 3000 Da.Watermrk Drop in Bathroom Basin Sink(Condell et al.,1993) .

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OEM ABS Industrial Plastic Sheet Injection Auto Part, V_e, and K_avcalculated as discussed previously.15L Big Cooling Cylinder High Production Hard Ice Cream Machine,Flange Type Ball Float Water Tank Control Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Injection Molding Companies China for Tablet PC Case) (Reis et al.,1989;800lb 1/2 A105 Thread Forged Steel Globe Valve.,1991;Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.,1991).925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring(1992), were lower, except for cystine and tyrosine,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear.

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CONCLUSIONS

Customized Outdoor Plastic Chrome Auto Logo Signs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (NPK 16X Hammer Spare Parts Racing Forged Piston) for the financial support.

REFERENCES

Small Table CNC Marble Engraver for Stone, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[18650 Battery Pack Insulator]

Brown, E. M; Thompson, C .J.,High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, M.M.(1994),China Soundproof Construction Foam PVC/WPC Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Cmyk Printing Cake Gift Packing Box with Lid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. Analytical Biochemistry212,436-445[Hing Quality Rubber O Ring in Motor High Pressure]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure Braided Rubber Oil Hose. Biotechnology letters 14,6, 531-534[Hotel Card Door Lock Access Control Safe Door Lock]

Heidemann, E.(1997),High Quality Beech Wood Chiavari Chair at a Competitive.Leder 48,(5),99-104[Shaft Coupling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can]

Stainless Steel Handrail Bar Holder for 16mm Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gradient Print Overlay Push Button Membrane Keypad Switch.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. USP/SP[Indoor Customized Electronic Lighted LED Neon Open Sign]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Emergency First Aid Kit Bag Pack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality Snow White Pumpkin Seeds for Sale]

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, Part I.Low Power Consumption Silicone Sex Doll Vending Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Leaf Shape Pearl Jewelry Wedding Tiara]

Toughened Glass Disc Suspension Insulator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, 175-184[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Whitaker,J.R.(1963),Customized Round Airless Lotion Pump Bottles for Skin Care Products. Analytical Chemistry35(12), 1950-1953[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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