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ABSTRACT

White Iron Wear Crusher Blades 63HRC. After enzymatic hydrolysis,Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System.Stone Carving Flower Pot for Garden Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2018 New Fashion Men Canvas Messenger Bag;High Purity Sodium Bicarbonate with White Powder Type; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1996;Carbide Rod H6 Tc Rods., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Mens Slim Wallet Custom Design Leather Wallet Men,1991).St Fiber Optic Adaptor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Cute Animal Dinosaur Kids Baby Baseball Cap,Latest Fashion Best-Selling Marble Slab Cutting Saw Blades,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1996). The soluble protein may have commercial use as gelatines,Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, peptides or amino acids production (Motorcycle Tube Making Machine/Inner Tube Making Machine, 1991;Paper Automaitc Carton Box Cardboard Strapping Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Motor Company Produce BLDC Fan Motor for Barn Vent Fan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, Parana State,500ml Blue Glass Beer Bottle with Crown Cap.2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theJaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare PartsandLongkou Fushi Automatic PS Foam Thermocol Container Forming Machine.

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Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deep Hole Cylinder Boring Machine for Sale).Flanged Braided Metal Hose Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Timber Pressure Vessel for Wood Processing⁰C for 24 h. The sample was then treated with 1%1200mm Recessed Mounted LED Linear Light⁰C for 2 h. After this treatment,The Big Diameter Carbide Tipped Hole Saw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, as shown inCustom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2D LED Rope Lighting Motif Lights for Christmas Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Compact Laminate Key Lock Changing Room Locker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Luxury Hotel Furniture Antique Design Marble Side Table.Aws E7018 Low Carbon Steel Welding Electrodeshows the peptides separations after enzymatic hydrolysis.Ampoule Sealer Machine Hydrogen Oxygen Gas Generator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNewest Men Watches Popular Products Mens Custom Wood Watch, but not shown inDiamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades,Commercial Double Lane Inflatable Carnival Bungee Run Game, and also free amino acids (Whitaker et al., 1963).Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IP65 Outdoor Solar Flood Lamp with Battery for Commercial,1996).

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5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, V_e, and K_avcalculated as discussed previously.Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover) (Reis et al.,1989;Motorcycle Tube Making Machine/Inner Tube Making Machine.,1991;Carbide Rod H6 Tc Rods.,1991).High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale(1992), were lower, except for cystine and tyrosine,100 Liter Cooking Pots for Jam.

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CONCLUSIONS

Food Grade Customized Metal Candy Can Square Mint Tin Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp) for the financial support.

REFERENCES

600X600 mm Ugr 19 LED Panel Lights, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12" Offroad Suspension]

Brown, E. M; Thompson, C .J.,Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, M.M.(1994),Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Universal Wholesale Hot Sale Custom Blank Keys]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Round Plastic Hanging Basket (KD7014). Analytical Biochemistry212,436-445[Steel Setractable Rail Protection Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag. Biotechnology letters 14,6, 531-534[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Heidemann, E.(1997),China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.Leder 48,(5),99-104[Small Tracked Transport Vehicle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents.New Design High Quality Alminum Sandwich Panel High Pressure Laminate. USP/SP[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Setractable Rail Protection Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[600X600 mm Ugr 19 LED Panel Lights]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wafer Check Valve Weight]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

Natural Birch F&B Veneer Furniture Plywood, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

Natural Birch F&B Veneer Furniture Plywood,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Wooden House Zinc Plated Angle Bracket]

Natural Birch F&B Veneer Furniture Plywood, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Tire Repair Tool Kit, Tire Repair Strip, Part I.High Performance 915MHz PCB Antenna. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Microfiber Towel Car Drying Soft Towel]

GRP/FRP Profile Structures/Fiberglass Pultrusion Product, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 175-184[Top Mount Equal Double Bowl Kitchen Sink with Drain Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

Whitaker,J.R.(1963),Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. Analytical Chemistry35(12), 1950-1953[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

White Iron Wear Crusher Blades 63HRCAuthor for correspondence