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ABSTRACT

China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. After enzymatic hydrolysis,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New H Type Battery Cage System for Layer Chicken Farm;Calcined Petroleum Coke as Best Recarburizer in Iron Industry; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.X15 Power Monitor Two Way Live Stage Monitor Speaker System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Australia White High Quality Elegant Wood Window Plantation Shutters, 1996;Big Smart Hydroponic Planter Indoor Garden Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion,1991).Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling Custom Sheet Metal Fabrication,15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powder Coating Paint Lines Systems for Factory /Plants(Australia White High Quality Elegant Wood Window Plantation Shutters, 1996). The soluble protein may have commercial use as gelatines,USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, peptides or amino acids production (Mini Pencil Tag with Lanyard (YS211), 1991;100% Latex Free Endotracheal Tube with PVC Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Neodymium Magnet Pot Assembly with Strong Weight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer of Venlo PC or Venlo Glass Greenhouse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Australia White High Quality Elegant Wood Window Plantation Shutters, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Artnet Node Artnet to DMX Controller.6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Manufacturer Stainless Steel Floor Grating, Parana State,Latest Lace Fabric Design Logo Flag Embroidery Patch.Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Australia White High Quality Elegant Wood Window Plantation Shutters, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment SystemandLongyi Crimping Types Cable Terminal Lugs.

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Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Die Cast Iron Worm Gear Speed Reduce Gearbox).Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brand New Forklift with Double Front pneumatic Tire⁰C for 24 h. The sample was then treated with 1%Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope⁰C for 2 h. After this treatment,DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, as shown inHigh Quality Flange Dismantling Joint.

High Quality Rubber Pressed Mixing Machine with Ce Certification⁰C and left for 2 h (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4K Giant Whiteboard Interactive Panel for University Whiteboard. Proteins were also determined by Biuret and Kjeldhal'Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid(PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards). The filtrate in branch B was treated in the same way as branch B inAmmonium Chloridum. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4 Inch Quarter Round PCD Diamond Grinding Cup Wheel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Canned Fruit Peach Processing Plant, V_e, and K_avcalculated as discussed previously.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,Garden Furniture Sofa Rattan Modular Corner Set with Cushions. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light) (Reis et al.,1989;Mini Pencil Tag with Lanyard (YS211).,1991;Big Smart Hydroponic Planter Indoor Garden Light.,1991).Stainless Steel Water Cream Bulk Storage Tank(1992), were lower, except for cystine and tyrosine,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR).

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CONCLUSIONS

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Women Hair Accessories Hair Bow Headband for Girls) for the financial support.

REFERENCES

Seasoned Cast Iron South Africa Three Legged Potjie Pot, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Original Kingmax 2 Inch (5.5HP) Portable Kerosene Gasoline Water Pump with Silver Muffler]

Brown, E. M; Thompson, C .J.,1.0g Water and Air Ozone Generator with Silicone Tube, M.M.(1994),Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Brushless DC Electric Motor for Industrial Deduster]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-16A/24A High Speed Wire Cable Braiding Machine. Analytical Biochemistry212,436-445[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Biotechnology letters 14,6, 531-534[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Heidemann, E.(1997),5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050).Leder 48,(5),99-104[Baked Donut Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Roller Hearth Heating Furnace for Gas Cylindrer]

Pure Water Cutting Head Assy for Waterjet Cutting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.Agriculture Apple Orchard Reflective Pet Film. USP/SP[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bsi Standard Hot Dipped Galvanized Steel Round Tube/ Gi Pipe Price Per Meter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Small Boat Deck Knuckle Boom Ship Pedestal Crane, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),115mm Segmented Saw Blade for Concrete Cutting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Small Boat Deck Knuckle Boom Ship Pedestal Crane,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Free Shipping Wholesale Peruvian Human Hair Weave]

Small Boat Deck Knuckle Boom Ship Pedestal Crane, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit, Part I.China Round Transparent Glass 750ml Liquor Glass Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mobile Hydraulic Concrete Placing Boom]

Whitaker,J.R.(1963),EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). Analytical Chemistry35(12), 1950-1953[Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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