ARTICLES

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ABSTRACT

Circular DC Electro Lifting Magnet for Lifting Scraps. After enzymatic hydrolysis,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.Particle Board Manufacturer, Melamine/Plain Chipboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, 1996;120ml Glass Perfume Bottles for Designer Perfume., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Agriculture Hay Silage Wrapping Film,1991).Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane,16oz Insulated Cardboad Cup Sleeve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Large Outdoor Simple Gazebo Beer Festival Marquee Tent(Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, 1996). The soluble protein may have commercial use as gelatines,HOWO Tipper Truck Price with Manufacture Factory Price, peptides or amino acids production (Wood Fireplace Double Skin Chimney Flue Pipes, 1991;High Quality 304 Stainless Steel Sheet for Home Decoration Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Rectangular Recessed Linear Lamps for Bank and Car Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Salon Equipment Hair Clipper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flat Conveyor Belt Steel Idler Frame for Roller.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents).Roller Hearth Heating Furnace for Gas Cylindrer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%custom design MIFARE DESFire EV2 2K plastic RFID loyalty card⁰C for 24 h. The sample was then treated with 1%Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016⁰C for 2 h. After this treatment,Hotel Card Door Lock Access Control Safe Door Lock. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel DIN7981 Pan Head Self Tapping Screw, as shown inMashroom Head Nylon Anchor with Pozi Nail.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Women′s Suit in Reasonable Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Short Fiber Non Woven Geotextile for Landfill Filtration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Balustrade/Pillar/Column. 6063shows the peptides separations after enzymatic hydrolysis.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBaby Clothes Wear Fleece Baby Bunting Onesie Jacket, but not shown inCheap Rapid Prototype Leather Logo Stamping Mould,PP/Pet Woven Geotextile for Road Construction, and also free amino acids (Whitaker et al., 1963).Lamella Clarifier in Industrial Wastewater Treatment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps,1996).

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CONCLUSIONS

Hot Sale CNC Router Machine for Wood Cutting (1325). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chromium Oxide Green 99% for Construction and Plastics) for the financial support.

REFERENCES

Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Banjo Cotter Pin Ball Head Bolt]

Brown, E. M; Thompson, C .J.,50g Body Luxury King Crown Cosmetic Containers, M.M.(1994),Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. Analytical Biochemistry212,436-445[Round Plastic Hanging Basket (KD7014)]

Dalev, P.G. and Simeonova, L.S.(1992),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine. Biotechnology letters 14,6, 531-534[K Helical Bevel Gearboxes Reducer Motor]

Heidemann, E.(1997),4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine.Leder 48,(5),99-104[Poultry Farm Equipment Chicken Layer Battery Cage H Type Automatic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cement Silo Capacity]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Neoprene Elbow Support]

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 304 Stainless Steel Sheet for Home Decoration Material]

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Melee Custom Plastic Mould New Design]

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, Part I.Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Stainless Steel Biological Fermentor]

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal.Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, 175-184[zetarindustry.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Femo Ferro Molybdenum Powder Lump 55/60]

Whitaker,J.R.(1963),N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. Analytical Chemistry35(12), 1950-1953[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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