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ABSTRACT

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). After enzymatic hydrolysis,CNC Metal Grinding Machine Tool Mk2120.Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, 1996;Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal,1991).Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet,Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale(Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, 1996). The soluble protein may have commercial use as gelatines,High Quaity China Supplier Truck Trailer Auto Car Accessories, peptides or amino acids production (Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, 1991;Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Steel Investment Casting High Hardness Agricultural Machinery Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Minimalist Slim Real Leather Front Pocket Card Wallet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Natural Cleaning Konjak Sponge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor Load Break Switch Isolating Switch.Biodegradable Disposible Straw Pulp Food Box Ppp-1007-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors).Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet⁰C for 24 h. The sample was then treated with 1%12 Inch King Mattress Latex Foam Memory Foam Mattress⁰C for 2 h. After this treatment,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, as shown inChina Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring.

Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable⁰C and left for 2 h (Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Longyi Crimping Types Cable Terminal Lugs. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rigid PVC Foam Board for FRP Marine. Proteins were also determined by Biuret and Kjeldhal'Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display(New Design Pet Product). The filtrate in branch B was treated in the same way as branch B inCreative Multilayer Shoe Rack Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Furniture Gold PVD Plasma Coating Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Bread Crumb Processing Line and Making Extruder Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Solid Tungsten Carbide Rods.120L 240L Oil Universal Chemical Spil Absorbent Kitsshows the peptides separations after enzymatic hydrolysis.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950, but not shown in1200X1000X810 Strong Collapsible Plastic Containers for Storage,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, and also free amino acids (Whitaker et al., 1963).High Tensisle Strength Tissue Paper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hydraulic Electric Stationary Car Platform Scissor Elevator Lift,1996).

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PP Coated Pipe Machine Extruder Machine, V_e, and K_avcalculated as discussed previously.China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sheet Metal Distribution Cabinet with High Quality) (Reis et al.,1989;Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.,1991;Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.,1991).Mining Machinery Parts Diamond Saw Blade Segments for Marble(1992), were lower, except for cystine and tyrosine,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface.

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CONCLUSIONS

Multi Column Load Cell 100kg, 20t. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap Wooden House Zinc Plated Angle Bracket) for the financial support.

REFERENCES

LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2017 Newest FHD1080p Car DVR Car Dash Camera]

Brown, E. M; Thompson, C .J.,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, M.M.(1994),Foldable PP Plastic Storage Logistics Turnover Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Artnet Node Artnet to DMX Controller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Custom Sheet Metal Fabrication]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Analytical Biochemistry212,436-445[Small Boat Deck Knuckle Boom Ship Pedestal Crane]

Dalev, P.G. and Simeonova, L.S.(1992),Buddhist Mood Picture Wallpaper Murals. Biotechnology letters 14,6, 531-534[Double Sides Clear White Frosted Acrylic Sheet]

Heidemann, E.(1997),High Quality Flange Dismantling Joint.Leder 48,(5),99-104[Perforated Mesh Type and Perforated Metal Mesh Speaker Grille]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Roller Skating Sneakers Shoes for Children (8083)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SMP-03 AC Mini Submersible Water Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Junior Backyard Training Practise Soccer Goal Net]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Paper Automaitc Carton Box Cardboard Strapping Machine]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, Part I.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cast Steel Dual Plate Wafer Check Valve]

Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kubota Combine Harvester Spare Parts 5h484-2392 Nut.Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, 175-184[Cpvc Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

Whitaker,J.R.(1963),HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor. Analytical Chemistry35(12), 1950-1953[16A/24A High Speed Wire Cable Braiding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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