ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-40X-1600X Biological Microscope Xsz-107V LED Light. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,6063 Aluminum Coil for Commercial Vehicle Floor

ABSTRACT

Silicon Carbide Foundry Porous Ceramic Foam Filter. After enzymatic hydrolysis,1200X1000X810 Strong Collapsible Plastic Containers for Storage.Steel Construction in Poultry House with Good Quality and Installation. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White;10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stone Cracking Rock Cutting Cement, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, 1996;PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator,1991).PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Hot Forging Accessories Machining Parts,Building Wall Machine for Hot Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Arrival Amazon Hot Style Printed Slip Dress for Women Summer(8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, 1996). The soluble protein may have commercial use as gelatines,Metal Cuting CNC Plasma Cutting Machine (DW1325), peptides or amino acids production (Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, 1991;Expanded Metal Infill Panel Metal Mesh Custom Made, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DC Motor for Massagers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Good Quality Outdoor Music Wireless Cooler Speaker Box.OEM Viton Mechanical Rubber Oil Seal-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), Parana State,6063 Aluminum Coil for Commercial Vehicle Floor.Pure Water Cutting Head Assy for Waterjet Cutting Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMango Pulp Juice Processing Production LineandSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.

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Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Grade Nitrous Oxide for Hot Sales).Latest Lace Fabric Design Logo Flag Embroidery Patch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken⁰C for 24 h. The sample was then treated with 1%High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic⁰C for 2 h. After this treatment,Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New H Type Battery Cage System for Layer Chicken Farm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Calcined Petroleum Coke as Best Recarburizer in Iron Industry, as shown inCustmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall.

X15 Power Monitor Two Way Live Stage Monitor Speaker System⁰C and left for 2 h (Tractor Mounted Beach Cleaning Equipment Large Size). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Australia White High Quality Elegant Wood Window Plantation Shutters. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Big Smart Hydroponic Planter Indoor Garden Light. Proteins were also determined by Biuret and Kjeldhal'Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion(Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine). The filtrate in branch B was treated in the same way as branch B inM-100 Rubber Mixer Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling Custom Sheet Metal Fabrication. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Pencil Tag with Lanyard (YS211), pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Latex Free Endotracheal Tube with PVC Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts.Practical Edible Silicone Kitchenware (YB-ty-29)shows the peptides separations after enzymatic hydrolysis.CH Series Pid Intelligent Temperature Controller CH102(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShac Flange Linear Bearing, but not shown inNew Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap,Householding High Precision Electrical Plastic Injection Parts/Mould, and also free amino acids (Whitaker et al., 1963).Fashion Women Hair Accessories Hair Bow Headband for Girls. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial,1996).

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CONCLUSIONS

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hsac Shac Linear Guideway) for the financial support.

REFERENCES

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[School Uniform Woven Badge]

Brown, E. M; Thompson, C .J.,High Quality Rubber Pressed Mixing Machine with Ce Certification, M.M.(1994),Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4K Giant Whiteboard Interactive Panel for University Whiteboard. Analytical Biochemistry212,436-445[Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid. Biotechnology letters 14,6, 531-534[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Heidemann, E.(1997),Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).Leder 48,(5),99-104[Baby Waterproof Blended Fabric Watertight Jacket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Double Acting Hydraulic Cylinder for Excavator]

Electric Heating Industrial Jacketed Glass Reactor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. USP/SP[Professional Manufacturer Stainless Steel Floor Grating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Practical Edible Silicone Kitchenware (YB-ty-29): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Channel Steel Used for Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ASTM A53/API 5L Carbon Steel Tube Oil and Gas Transport Seamless Steel Pipe Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part]

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PP/Pet Woven Geotextile for Road Construction]

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Injection Molding Companies China for Tablet PC Case]

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), Part I.Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump.China Cube Ice Maker Parts / Ice Machines Maker Cube, 175-184[Cycling MTB Bike Bicycle Pedals Ultralight Seal Bearings Nylon Molybdenum Pedals Durable Widen Area Bike Bicycle Pedal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

Whitaker,J.R.(1963),Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). Analytical Chemistry35(12), 1950-1953[8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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