ARTICLES

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ABSTRACT

Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid. After enzymatic hydrolysis,Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1996;Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl,1991).99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump,China Cube Ice Maker Parts / Ice Machines Maker Cube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)(High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1996). The soluble protein may have commercial use as gelatines,3D Adjustable American Market Cabinet Door Mini Hinge, peptides or amino acids production (Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, 1991;Custom Design 100% Silk Tie for Men, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Om4 Sc/Upc-LC/Upc Fiber Optic Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Nylon Back to Back Hook&Loop.Canned Fruit Peach Processing Plant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Seasoned Cast Iron South Africa Three Legged Potjie Pot, Parana State,4K Giant Whiteboard Interactive Panel for University Whiteboard.1.0g Water and Air Ozone Generator with Silicone Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEnviornment-Friendly Broken Stone Pattern Exterior Wall Decorative PanelsandHigh Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04).

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Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes).16A/24A High Speed Wire Cable Braiding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot⁰C for 24 h. The sample was then treated with 1%5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)⁰C for 2 h. After this treatment,20 Inch 48V 750W Fat Tire Electric Bike with Double Seat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Agriculture Apple Orchard Reflective Pet Film's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), as shown inHot Sale Dental Material Dental PMMA Block From China.

Small Boat Deck Knuckle Boom Ship Pedestal Crane⁰C and left for 2 h (Precision Customized CNC Machined Mild Steel Flange for Automobile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.115mm Segmented Saw Blade for Concrete Cutting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. Proteins were also determined by Biuret and Kjeldhal'China Round Transparent Glass 750ml Liquor Glass Bottle(Calcined Petroleum Coke as Best Recarburizer in Iron Industry). The filtrate in branch B was treated in the same way as branch B inm.tianantextiles.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3D Creative Design Metal Medal Promotion Gift of Sport.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ad-L13 Black Perfume Plastics Vial Bottle 10ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPatchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Pushbutton Ultrasonic Spin Welding MachineandMexico Single Expansion Anchor with YZP. Enzymatic (Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe)SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet(sulfuric acid)Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Price 4X6 Acrylic Magnetic Photo Frame.Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elementsshows the peptides separations after enzymatic hydrolysis.200m Diesel Oil Hydraulic Water Well Drilling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMotorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, but not shown in6063 Aluminum Coil for Commercial Vehicle Floor,Custom Cmyk Printing Cake Gift Packing Box with Lid, and also free amino acids (Whitaker et al., 1963).Air Purification Activated Alumina. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine,1996).

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FRP Pultruded Pipe or GRP Profile, V_e, and K_avcalculated as discussed previously.6090 CO2 Sealed Glass Cooling Laser Cutting Machine,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wood Activated Carbon for Decolorizing in Food Industry with ASTM) (Reis et al.,1989;Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.,1991;Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG).,1991).Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper(1992), were lower, except for cystine and tyrosine,Outdoor Fitness Equipment Swing (JMH-03).

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CONCLUSIONS

Sheet Metal Distribution Cabinet with High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)) for the financial support.

REFERENCES

OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zenithwirl]

Brown, E. M; Thompson, C .J.,New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, M.M.(1994),Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. Analytical Biochemistry212,436-445[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed HDMI Cable Wholesale HDMI with Ethernet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Wholesale Oxide Aluminium Black Sand Blast Media: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ SLA Shoes Prototyping for Auto Accessory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Italy Design Super Light Fashion Cat Eye Acetate Frame Metal Plastic Bamboo Wood Eyewear Vintage Shiny Woman Sports Party Polarized Sunglasses with Nylon Lens]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Welded Stud Anchor Chain for Ship, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sludge Oil Separation Centrifuge]

Welded Stud Anchor Chain for Ship,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Welded Stud Anchor Chain for Ship, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Line Scan Uvss System Portable Under Vehicle Surveillance, Part I.Folding Steel Manual Wheelchair with Chrome Frame. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Real Leather Wallet Mens Designer Leather Wallets]

Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Style Lady Alloy Quartz Watch with Simple Dial.Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 175-184[China Best Acupressure Mat Reviews]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

Whitaker,J.R.(1963),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. Analytical Chemistry35(12), 1950-1953[ 60 Hz]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stone Cracking Rock Cutting CementAuthor for correspondence