ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. After enzymatic hydrolysis,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).Stainless Steel Furniture Gold PVD Plasma Coating Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment;Steel Pipe Rust Removal Shot Blasting Cleaning Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, 1996;Automatic Bread Crumb Processing Line and Making Extruder Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder,1991).China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve,Plastic Injection Molding Companies China for Tablet PC Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools(2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, 1996). The soluble protein may have commercial use as gelatines,High Quality Solid Tungsten Carbide Rods, peptides or amino acids production (Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, 1991;Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Tensisle Strength Tissue Paper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tractor Mounted Beach Cleaning Equipment Large Size.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.PP Coated Pipe Machine Extruder Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, Parana State,Rigid PVC Foam Board for FRP Marine.Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC Powder Coated Galvanized Metal Welded Wire Mesh FenceandWholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.

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Foldable PP Plastic Storage Logistics Turnover Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Multifunctional Solar Power Flashlight (SYSG-517)).China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Buddhist Mood Picture Wallpaper Murals⁰C for 24 h. The sample was then treated with 1%High Quality Flange Dismantling Joint⁰C for 2 h. After this treatment,Duplex Stainless Steel Floating Ball Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, as shown inCross Hex Flange Screw.

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple⁰C and left for 2 h (Factory Small Coil Compression Spring in High Quality). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. Proteins were also determined by Biuret and Kjeldhal'7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)). The filtrate in branch B was treated in the same way as branch B inGlass Drinking Wide Mouth Mason Jar with Handle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kubota Combine Harvester Spare Parts 5h484-2392 Nut. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Floor Deck Post Cutting Cold Roll Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fluorous Rubber Coated Fiberglass Cloth, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Brushless DC Electric Motor for Industrial Deduster. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface) for the financial support.

REFERENCES

China Factory Light Soda Ash /Sodium Carbonate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Shoe Box Stainless Steel Storage Locker]

Brown, E. M; Thompson, C .J.,Regular Size Cigarette Rolling Papers, M.M.(1994),304L Stainless Steel U Channel Good Performance.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Machining Metal Mechanical Engineering Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). Analytical Biochemistry212,436-445[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Dalev, P.G. and Simeonova, L.S.(1992),Junior Backyard Training Practise Soccer Goal Net. Biotechnology letters 14,6, 531-534[50g Body Luxury King Crown Cosmetic Containers]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Mold Manufacturer Plastic Injection Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laser ID Card Rigid PET Printing Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[WPC Wood Plastic Board Equipment Extruder Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional CNC Machining Parts with Aluminum/Brass/Stainless Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[40X-1600X Biological Microscope Xsz-107V LED Light]

New Arrival Plush Animal Ride for Kids for Shopping Center, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

New Arrival Plush Animal Ride for Kids for Shopping Center,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting]

New Arrival Plush Animal Ride for Kids for Shopping Center, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, Part I.Ss316 Stainless Steel Heavy Duty Straps. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Tire Repair Strip]

6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Temperature Side Opening AAC Autoclave, AAC Block Making Machine.PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, 175-184[Recycling Plant to Shred Waste/Scrap/Used Tyre Into Rubber Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

Whitaker,J.R.(1963),Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Analytical Chemistry35(12), 1950-1953[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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