ARTICLES

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ABSTRACT

OEM Viton Mechanical Rubber Oil Seal. After enzymatic hydrolysis,Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric.Q235 Q345 Hot Rolled Channel Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 1996;Pure Water Cutting Head Assy for Waterjet Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat,1991).Latest Lace Fabric Design Logo Flag Embroidery Patch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05(Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 1996). The soluble protein may have commercial use as gelatines,New H Type Battery Cage System for Layer Chicken Farm, peptides or amino acids production (Calcined Petroleum Coke as Best Recarburizer in Iron Industry, 1991;X15 Power Monitor Two Way Live Stage Monitor Speaker System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Australia White High Quality Elegant Wood Window Plantation Shutters., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Big Smart Hydroponic Planter Indoor Garden Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire.Hot Selling Custom Sheet Metal Fabrication-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Neodymium Magnet Pot Assembly with Strong Weight, Parana State,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box.Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHiwin Mgn Series Linear Guide Rail and Slide Block Mgn9hand Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller.

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China Manufacturer of Venlo PC or Venlo Glass Greenhouse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery).Artnet Node Artnet to DMX Controller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes⁰C for 24 h. The sample was then treated with 1%Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts⁰C for 2 h. After this treatment,CH Series Pid Intelligent Temperature Controller CH102. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Householding High Precision Electrical Plastic Injection Parts/Mould's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Women Hair Accessories Hair Bow Headband for Girls, as shown inCsm DIY Microscope for Kids/Toy Microscope.

Round Hole Carbon Steel Pipe Perforated for Filter⁰C and left for 2 h (Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Manufacturer Stainless Steel Floor Grating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels. Proteins were also determined by Biuret and Kjeldhal'Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose(High Quality Electronic Circuit Board Supplier PCB Assembly). The filtrate in branch B was treated in the same way as branch B inluqi-tech. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brand New Forklift with Double Front pneumatic Tire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Rubber Pressed Mixing Machine with Ce Certification, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can.Duplex Stainless Steel Floating Ball Valveshows the peptides separations after enzymatic hydrolysis.Practical Edible Silicone Kitchenware (YB-ty-29)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlexible Vj Rigid Shaft Coupling for Pipe Flange, but not shown in10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents,4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, and also free amino acids (Whitaker et al., 1963).High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10),1996).

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Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, V_e, and K_avcalculated as discussed previously.China Cube Ice Maker Parts / Ice Machines Maker Cube,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Baby Clothes Wear Fleece Baby Bunting Onesie Jacket) (Reis et al.,1989;Calcined Petroleum Coke as Best Recarburizer in Iron Industry.,1991;Pure Water Cutting Head Assy for Waterjet Cutting Machine.,1991).3D Adjustable American Market Cabinet Door Mini Hinge(1992), were lower, except for cystine and tyrosine,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.

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CONCLUSIONS

Custom Design 100% Silk Tie for Men. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Pencil Tag with Lanyard (YS211)) for the financial support.

REFERENCES

Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[tiger-water-filter]

Brown, E. M; Thompson, C .J.,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, M.M.(1994),Om4 Sc/Upc-LC/Upc Fiber Optic Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kubota Combine Harvester Spare Parts 5h484-2392 Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Nylon Back to Back Hook&Loop. Analytical Biochemistry212,436-445[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Dalev, P.G. and Simeonova, L.S.(1992),Canned Fruit Peach Processing Plant. Biotechnology letters 14,6, 531-534[Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump]

Heidemann, E.(1997),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.Leder 48,(5),99-104[Syringe Pump Medical Injection Pump with Double/Single Channel Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel]

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Garden Furniture Sofa Rattan Modular Corner Set with Cushions.Stainless Steel Water Cream Bulk Storage Tank. USP/SP[Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Batch Glass Lined Chemical Reactor with Water Heating or Cooling Jacket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts]

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Seasoned Cast Iron South Africa Three Legged Potjie Pot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Plastic Hanging Basket (KD7014)]

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[K Helical Bevel Gearboxes Reducer Motor]

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.0g Water and Air Ozone Generator with Silicone Tube, Part I.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

16A/24A High Speed Wire Cable Braiding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 175-184[355mm Pn10 PVC Rubber Ring Joint Coupling (F/F)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Nylon Back to Back Hook&Loop]

Whitaker,J.R.(1963),20 Inch 48V 750W Fat Tire Electric Bike with Double Seat. Analytical Chemistry35(12), 1950-1953[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

DC 12V 24V Mini Portable Car Refrigerator Compressor FridgeAuthor for correspondence