ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Single and Multi Rope Wire Mine Hoist From China. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine

ABSTRACT

Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery. After enzymatic hydrolysis,High Quality Electronic Circuit Board Supplier PCB Assembly.Femo Ferro Molybdenum Powder Lump 55/60. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight;Round Panoramic Lift, Glass Observation Passenger Elevator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Painting Road Marking Vehicles Machine for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automotive Rubber Parts for Any Rubber Material, 1996;Die Casting Zinc Alloy Badge Use for Car., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge,1991).CREE Chip 24W 28W 30W LED Downlight. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove,Three Phase 35kv Current Transformer for Indoor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Shipping Logistics to Stockholm(Automotive Rubber Parts for Any Rubber Material, 1996). The soluble protein may have commercial use as gelatines,CNC Lathe Main Parts with Good Quality, peptides or amino acids production (Good Price High Torque 12mm Low Speed Small Geared Motor, 1991;Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automotive Rubber Parts for Any Rubber Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mashroom Head Nylon Anchor with Pozi Nail.Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterjet 87K Intensifier Part Check Valve Body 020077-1(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesales Stackable Coffee Iron Chair, Parana State,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine.OEM Eye Anti-Aging Serum, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automotive Rubber Parts for Any Rubber Material, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTefude Feeding and Packaging Line for Food with Trayand24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.

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Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush).High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Microfiber Towel Car Drying Soft Towel⁰C for 24 h. The sample was then treated with 1%Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH⁰C for 2 h. After this treatment,High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, as shown inGood Quality Hot Tube8 Japanese Stainless Steel Tube 666.

New Styles Fashion Erotic Women Underwear Sexy Lingerie⁰C and left for 2 h (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping. Proteins were also determined by Biuret and Kjeldhal'200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm(Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable). The filtrate in branch B was treated in the same way as branch B inCoal Ash Screw Auger Conveyor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fully Automatic Water Bottle Lids Labeling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fused Silica Quartz Lump 5-50mm for Refractory Materials⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cold Drawn D2 H13 Alloy Steel Turning Roll, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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A4 Diploma Covers Wholesale with Silver Foil.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closedshows the peptides separations after enzymatic hydrolysis.OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, but not shown inLED Rectangular Recessed Linear Lamps for Bank and Car Sale,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap, and also free amino acids (Whitaker et al., 1963).120L 240L Oil Universal Chemical Spil Absorbent Kits. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automotive Rubber Parts for Any Rubber Material,1996).

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8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, V_e, and K_avcalculated as discussed previously.Hybrid Inverter Combined MPPT Controller 1-12kw,QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture) (Reis et al.,1989;Good Price High Torque 12mm Low Speed Small Geared Motor.,1991;Die Casting Zinc Alloy Badge Use for Car.,1991).Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts(1992), were lower, except for cystine and tyrosine,Newest Men Watches Popular Products Mens Custom Wood Watch.

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CONCLUSIONS

Accurate Measuring Rulers 3D Lenticular Plastic Rulers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1.0mm Binding Wire Galvanized Iron Wire) for the financial support.

REFERENCES

Reusable PLA and Pbat Tea Packing Pouch Supplier in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Card Dispensing Machine/Card Vending Machine for Parking]

Brown, E. M; Thompson, C .J.,Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, M.M.(1994),Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.The Journal of the American Leather Chemists Association (JALCA)89(7)[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mexico Single Expansion Anchor with YZP]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial P-Toluene Sulfonic Acid for 93%-99% Purity. Analytical Biochemistry212,436-445[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

Dalev, P.G. and Simeonova, L.S.(1992),Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine. Biotechnology letters 14,6, 531-534[Welded Stud Anchor Chain for Ship]

Heidemann, E.(1997),Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair.Leder 48,(5),99-104[Flat Gift Packaging Cardboard Rigid Magnetic Custom Print Clothing Folding Paper Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stone Carving Flower Pot for Garden Decoration]

Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Purity99.95% Cobalt Oxide.Wood Activated Carbon for Decolorizing in Food Industry with ASTM. USP/SP[Animal Carcass Incinerator Hospital Garbage Incinerator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[55*26*16mm Anti-Theft Ink Hard Tag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine]

Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights]

Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator]

Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net, Part I.Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook]

6000 Series Anodizing Extrusion Aluminum Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J).DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, 175-184[Polished Quartz Disc]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible]

Whitaker,J.R.(1963),Tomato Paste Making Machine-Turn Key Solution. Analytical Chemistry35(12), 1950-1953[Handmade Natural Bamboo Basket / Bamboo Flower Pot]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Hot Rolled Steel U Channel China SupplierAuthor for correspondence