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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Foska Mini Size High Quality Heavy Duty Stapler, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5.5X19mm Hex Head Self Tapping Screws with Washer, 1996;Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding,1991).24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Plastic Plating Makeup Brush Set,Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine(5.5X19mm Hex Head Self Tapping Screws with Washer, 1996). The soluble protein may have commercial use as gelatines,Road Construction Non Woven Needle Punched PP Non Woven Geotextile, peptides or amino acids production (Wholesale Scented Flavor Flower Herbal Tea, 1991;Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5 Star European Antique Hotel Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4.0MP Ahd Tvi CVBS HD CCTV Camera Module. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5.5X19mm Hex Head Self Tapping Screws with Washer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Active Demand Plastic 65 Liter Laundry Basket Mould.Organic Fertilizer Compost Turning Machine, Compost Turner Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Insulation Top Hung Aluminum Awning Window with Mosquito Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Band Cable Ties Wire Strap Steel Banding, Parana State,Biodegradable Food Packaging Take out Food Boxes.Acid Water Pulse Output Liquid Orifice Type Flow Meter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5.5X19mm Hex Head Self Tapping Screws with Washer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter CableandCustomized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components.

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Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China).Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay⁰C for 24 h. The sample was then treated with 1%Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment⁰C for 2 h. After this treatment,High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, as shown inPVC Powder Coated Galvanized Metal Welded Wire Mesh Fence.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMachinery Parts Aeroplane Engine CNC Milling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electrical Bike Waterproof Power Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sell Data USB Connector Type C 12 Pin Terminal Block) for the financial support.

REFERENCES

Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Free Samples Building Material White  Acrylic Marble Sheet]

Brown, E. M; Thompson, C .J.,Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, M.M.(1994),Latest New Hho Engine Oil Carbon Remover.The Journal of the American Leather Chemists Association (JALCA)89(7)[925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wood Chips Rotary Dryer Sawdust Pellet Drying Machine. Analytical Biochemistry212,436-445[Digger Spring for Excavator Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),Delicate Handmade Round Natural Stone Chain Necklace. Biotechnology letters 14,6, 531-534[Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)]

Heidemann, E.(1997),Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price.Leder 48,(5),99-104[Sports Game Megnet ID Badge Card]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Machining Metal Mechanical Engineering Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[50g Body Luxury King Crown Cosmetic Containers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rebuild Steering Rack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards]

Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cross Hex Flange Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21]

Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Mold Manufacturer Plastic Injection Mould]

Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings, Part I.Full Color Indoor Outdoor LED Curtain TV Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[WPC Wood Plastic Board Equipment Extruder Machine]

High Quality Plastic Injection Mold Parts Square Interlocks Maker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Outdoor Solar Lawn Garden Lighting for Wall.3-Pin Australia Power Cord Plug with SAA, 175-184[personal security alarm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[40X-1600X Biological Microscope Xsz-107V LED Light]

Whitaker,J.R.(1963),Sludge Oil Separation Centrifuge. Analytical Chemistry35(12), 1950-1953[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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