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ABSTRACT

Oak Veneer Laminated Plywood for Decoration. After enzymatic hydrolysis,China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib.Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Women′s Suit in Reasonable Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet Short Fiber Non Woven Geotextile for Landfill Filtration, 1996;Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Competitive Price Sodium Gluconate as Concrete Admixture,1991).Precision Investment Casting Stainless Steel 304 Wire Rope Hook. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture,Custom Die Casting Aluminium Product for Connector Usage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Balustrade/Pillar/Column(Pet Short Fiber Non Woven Geotextile for Landfill Filtration, 1996). The soluble protein may have commercial use as gelatines,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, peptides or amino acids production (PP/Pet Woven Geotextile for Road Construction, 1991;Lamella Clarifier in Industrial Wastewater Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet Short Fiber Non Woven Geotextile for Landfill Filtration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick.Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IS Type Cast Iron Chemical Industrial Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

50g Body Luxury King Crown Cosmetic Containers, Parana State,Csm DIY Microscope for Kids/Toy Microscope.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pet Short Fiber Non Woven Geotextile for Landfill Filtration, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Suppliers Plastic Vacuum Blister Packaging Machine with Servo MotorandFactory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.

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Mini Succulent Planters, Cactus Pots with Wooden Cement Pot(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Painting Road Marking Vehicles Machine for Sale).Paper Egg Box Forming Molding Production Line/ Egg Tray Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine⁰C for 24 h. The sample was then treated with 1%Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)⁰C for 2 h. After this treatment,CNC Metal Grinding Machine Tool Mk2120. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, as shown in Wire.

Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM⁰C and left for 2 h (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. Proteins were also determined by Biuret and Kjeldhal'Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building(Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel). The filtrate in branch B was treated in the same way as branch B in20FT Side Open Storage Container 40FT Self Storage Container. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avN52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quaity China Supplier Truck Trailer Auto Car Accessories, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Biodegradable Disposible Straw Pulp Food Box Ppp-1007.QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machineryshows the peptides separations after enzymatic hydrolysis.T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, but not shown inYCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable,Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment, and also free amino acids (Whitaker et al., 1963).NBR Latex Adhesive for Glove. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pet Short Fiber Non Woven Geotextile for Landfill Filtration,1996).

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Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment SubstituteandFactory Supply Coaxial Cable Rg 59 with Messengershow the gel chromatographic separation of soluble protein with Sephadex G-Cheap Wooden House Zinc Plated Angle Bracket.4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, and another peak at 45 mL not shown inWholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, for peptides lower than 3000 Da.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box(Condell et al.,1993) .

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CONCLUSIONS

A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale CNC Router Machine for Wood Cutting (1325)) for the financial support.

REFERENCES

HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall]

Brown, E. M; Thompson, C .J.,Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, M.M.(1994),Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Chic Straw Braid Floppy Hat with Print Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[NBR Latex Adhesive for Glove]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Longyi Crimping Types Cable Terminal Lugs. Analytical Biochemistry212,436-445[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

Dalev, P.G. and Simeonova, L.S.(1992),Rigid PVC Foam Board for FRP Marine. Biotechnology letters 14,6, 531-534[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Heidemann, E.(1997),Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.Leder 48,(5),99-104[qj-ec.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Electric Piston Pump Coating Machine PT6900]

Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).Stainless Steel Furniture Gold PVD Plasma Coating Machine. USP/SP[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1200mm Recessed Mounted LED Linear Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Zinc Alloy Die Casting Gears]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Steel Pipe Rust Removal Shot Blasting Cleaning Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hydraulic Press Corrugated Cold Roll Automatically Forming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Crusher for Used Plastic Recycling Line]

Steel Pipe Rust Removal Shot Blasting Cleaning Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Accessories Fishing Hunting Masks]

Steel Pipe Rust Removal Shot Blasting Cleaning Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, Part I.Automatic Bread Crumb Processing Line and Making Extruder Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring.300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, 175-184[DN800 Large Pipe Petroleum Irrigation Water Drainage and Supply Digital Electromagnetic Flow Meter MFE600]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Sulphur Calcined Carbon Raiser]

Whitaker,J.R.(1963),Plastic Injection Molding Companies China for Tablet PC Case. Analytical Chemistry35(12), 1950-1953[12V 24V 220V Digital Temperature Controller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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