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ABSTRACT

World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J). After enzymatic hydrolysis,DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W.Tomato Paste Making Machine-Turn Key Solution. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper;Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HPMC Similiar to Tylose Powder for Rapid-Set Adhesive, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1996;a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Solidwood Viola Wholesale Price Viola,1991).Building Material Sound Heat Insulation Rock Wool Sandwich Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration,Ltmg Classic Product 6 Ton Wheel Front Loader for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror(NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1996). The soluble protein may have commercial use as gelatines,Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, peptides or amino acids production (Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, 1991;Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Household Family Medical Emergency Electronic Digital Thermometer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Die Cast Iron Worm Gear Speed Reduce Gearbox.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Couple Watches Steel Material Gift Watch OEM in China.Glass Tea Sugar Coffee Storage Jar with Screw Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Circular DC Electro Lifting Magnet for Lifting Scraps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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120ml Glass Perfume Bottles for Designer Perfume(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Big Bussiness Project Commerical Chicken Poultry Farm Buildings).Agriculture Hay Silage Wrapping Film(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer⁰C for 24 h. The sample was then treated with 1%Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane⁰C for 2 h. After this treatment,16oz Insulated Cardboad Cup Sleeve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Large Outdoor Simple Gazebo Beer Festival Marquee Tent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HOWO Tipper Truck Price with Manufacture Factory Price, as shown inCustom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card.

Wood Fireplace Double Skin Chimney Flue Pipes⁰C and left for 2 h (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 304 Stainless Steel Sheet for Home Decoration Material. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. Proteins were also determined by Biuret and Kjeldhal'LED Rectangular Recessed Linear Lamps for Bank and Car Sale(High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)). The filtrate in branch B was treated in the same way as branch B inCryogenic Temperatures to Extract Thc Oil From Cannabis and Cbd Oil From Hemp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Salon Equipment Hair Clipper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flat Conveyor Belt Steel Idler Frame for Roller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Durable TPR Pet Molar Toy with Colorful Rope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chromium Oxide Green 99% for Construction and Plastics, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

SSAW Steel Pipe API 5L Psl2 with Stainless Steel SheetandZs Round Vibrating Sieve. Enzymatic (Stainless Steel Tactile Paving Blind Road Stud)70L Lab Use Refrigerated Circulator Chiller Factory Price(sulfuric acid)Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)(NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Roller Hearth Heating Furnace for Gas Cylindrer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Roller Hearth Heating Furnace for Gas Cylindrer, flow rate of 0.1 mL/min., and l = 620 nm.

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card.Fully Automatic Water Bottle Lids Labeling Machineshows the peptides separations after enzymatic hydrolysis.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody, but not shown inScrew/Countersunk Head Screw 6 Ribs with Wing,Hotel Card Door Lock Access Control Safe Door Lock, and also free amino acids (Whitaker et al., 1963).Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple,1996).

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Dark Green Decorative Garden Welded Wire Mesh Fencing, V_e, and K_avcalculated as discussed previously.Csm DIY Microscope for Kids/Toy Microscope,Oak Veneer Laminated Plywood for Decoration. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Metric Female Cone Hydraulic Hose Fitting) (Reis et al.,1989;Samsung LTI550HN16 1920X1080P monitor screen LCD video wall.,1991;a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine.,1991).China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib(1992), were lower, except for cystine and tyrosine,Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.

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CONCLUSIONS

High Speed HDMI Cable Wholesale HDMI with Ethernet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part) for the financial support.

REFERENCES

Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Face Beauty Prefilled Syringe Hyaluronic Acid Face Fillers Cheek Dermal Fillers for Plastic Surgery]

Brown, E. M; Thompson, C .J.,High Quality Women′s Suit in Reasonable Price, M.M.(1994),Pet Short Fiber Non Woven Geotextile for Landfill Filtration.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Lathe Main Parts with Good Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. Analytical Biochemistry212,436-445[Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)]

Dalev, P.G. and Simeonova, L.S.(1992),Competitive Price Sodium Gluconate as Concrete Admixture. Biotechnology letters 14,6, 531-534[Full Color Indoor Outdoor LED Curtain TV Display]

Heidemann, E.(1997),Precision Investment Casting Stainless Steel 304 Wire Rope Hook.Leder 48,(5),99-104[Automatic Potato French Fries Production Line Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hybrid Inverter Combined MPPT Controller 1-12kw]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Balustrade/Pillar/Column: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Women Hair Accessories Hair Bow Headband for Girls]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[304/304L 316/316L Seamless Stainless Steel Pipe for Heat Exchanger]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PP/Pet Woven Geotextile for Road Construction-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine]

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Cnice Brand Toothpaste with Oral Refreshing]

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lamella Clarifier in Industrial Wastewater Treatment, Part I.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Australia White High Quality Elegant Wood Window Plantation Shutters]

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, 175-184[Teflon Tape with Competitive Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Shipping Logistics to Stockholm]

Whitaker,J.R.(1963),Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts. Analytical Chemistry35(12), 1950-1953[Cleanroom White PVC Dotted Anti-Static Gloves]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Metal Sheet Fabrication Hexagon Hole Punching with Galvanized SurfaceAuthor for correspondence