ARTICLES

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ABSTRACT

Electric Heating Industrial Jacketed Glass Reactor. After enzymatic hydrolysis,40X-1600X Biological Microscope Xsz-107V LED Light.6063 Aluminum Coil for Commercial Vehicle Floor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Silicon Carbide Foundry Porous Ceramic Foam Filter;1200X1000X810 Strong Collapsible Plastic Containers for Storage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Construction in Poultry House with Good Quality and Installation, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, 1996;10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stone Cracking Rock Cutting Cement,1991).8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards,Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag(Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, 1996). The soluble protein may have commercial use as gelatines,Aluminum Hot Forging Accessories Machining Parts, peptides or amino acids production (Building Wall Machine for Hot Machine, 1991;New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Cuting CNC Plasma Cutting Machine (DW1325)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Expanded Metal Infill Panel Metal Mesh Custom Made. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC Motor for Massagers.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Factory New Girls Nuts Crunch Cheap Price, Parana State,Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder.Hsac Shac Linear Guideway, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theE26 E14 E17 E17 E39 Lamp Cap Go No Go GaugeandUnbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.

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Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding).Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pure Water Cutting Head Assy for Waterjet Cutting Machine⁰C for 24 h. The sample was then treated with 1%Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat⁰C for 2 h. After this treatment,Latest Lace Fabric Design Logo Flag Embroidery Patch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, as shown inRound Panoramic Lift.

Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05⁰C and left for 2 h (Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New H Type Battery Cage System for Layer Chicken Farm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Calcined Petroleum Coke as Best Recarburizer in Iron Industry. Proteins were also determined by Biuret and Kjeldhal'X15 Power Monitor Two Way Live Stage Monitor Speaker System(Flange Type Ball Float Water Tank Control Valve). The filtrate in branch B was treated in the same way as branch B inChina Glass Web Suppliers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Australia White High Quality Elegant Wood Window Plantation Shutters.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Big Smart Hydroponic Planter Indoor Garden Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFluorine Lined Butterfly Valve Flanged Type Anti Corrosion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Custom Sheet Metal Fabrication⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Powder Coating Paint Lines Systems for Factory /Plants, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Manufacturer of Venlo PC or Venlo Glass Greenhouse.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camerashows the peptides separations after enzymatic hydrolysis.Artnet Node Artnet to DMX Controller(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inViscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, but not shown inPackaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500),6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, and also free amino acids (Whitaker et al., 1963).Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White,1996).

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Round Hole Carbon Steel Pipe Perforated for Filter, V_e, and K_avcalculated as discussed previously.Professional Manufacturer Stainless Steel Floor Grating,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part) (Reis et al.,1989;Building Wall Machine for Hot Machine.,1991;10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle.,1991).Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose(1992), were lower, except for cystine and tyrosine,Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips.

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CONCLUSIONS

Brand New Forklift with Double Front pneumatic Tire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric) for the financial support.

REFERENCES

Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Full-Automatic Laundry Machine Tumble Dryer (15kg to 100kg) for Sale]

Brown, E. M; Thompson, C .J.,DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, M.M.(1994),Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).The Journal of the American Leather Chemists Association (JALCA)89(7)[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity. Analytical Biochemistry212,436-445[8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Rubber Pressed Mixing Machine with Ce Certification. Biotechnology letters 14,6, 531-534[Custom Cmyk Printing Cake Gift Packing Box with Lid]

Heidemann, E.(1997),Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600.Leder 48,(5),99-104[Portable Mechanical Key Lock Safe Boxes Black Deposit Safe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hing Quality Rubber O Ring in Motor High Pressure]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor Customized Electronic Lighted LED Neon Open Sign]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[with Inspection Certificate Aluminized Paper for Wet Towels/ Glasses Wet Dry Wipes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Snow White Pumpkin Seeds for Sale]

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Practical Edible Silicone Kitchenware (YB-ty-29), Part I.4 Inch Quarter Round PCD Diamond Grinding Cup Wheel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG).Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, 175-184[Zhuzhou Sant Cutting Tools High Precision Indexable Internal Threading Toolholder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Whitaker,J.R.(1963),99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). Analytical Chemistry35(12), 1950-1953[New Leaf Shape Pearl Jewelry Wedding Tiara]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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