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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4K Giant Whiteboard Interactive Panel for University Whiteboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, 1996;Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting,1991).Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Practical Edible Silicone Kitchenware (YB-ty-29),4 Inch Quarter Round PCD Diamond Grinding Cup Wheel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine(Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, 1996). The soluble protein may have commercial use as gelatines,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), peptides or amino acids production (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, 1991;99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Cube Ice Maker Parts / Ice Machines Maker Cube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D Adjustable American Market Cabinet Door Mini Hinge.Factory Sale Various Customized Square 6063 Extruded Aluminum Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Design 100% Silk Tie for Men(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Garden Furniture Sofa Rattan Modular Corner Set with Cushions(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed HDMI Cable Wholesale HDMI with Ethernet).Stainless Steel Water Cream Bulk Storage Tank(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)⁰C for 24 h. The sample was then treated with 1%Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants⁰C for 2 h. After this treatment,Seasoned Cast Iron South Africa Three Legged Potjie Pot. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.0g Water and Air Ozone Generator with Silicone Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, as shown in SLA Shoes Prototyping for Auto Accessory.

16A/24A High Speed Wire Cable Braiding Machine⁰C and left for 2 h (Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). Proteins were also determined by Biuret and Kjeldhal'20 Inch 48V 750W Fat Tire Electric Bike with Double Seat(Sludge Oil Separation Centrifuge). The filtrate in branch B was treated in the same way as branch B inAluminum Curtain Wall System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Agriculture Apple Orchard Reflective Pet Film.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Boat Deck Knuckle Boom Ship Pedestal Crane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Round Transparent Glass 750ml Liquor Glass Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Creative Design Metal Medal Promotion Gift of Sport, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.Real Leather Wallet Mens Designer Leather Walletsshows the peptides separations after enzymatic hydrolysis.Mexico Single Expansion Anchor with YZP(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, but not shown in 60 Hz,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, and also free amino acids (Whitaker et al., 1963).SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid,1996).

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200m Diesel Oil Hydraulic Water Well Drilling Machine, V_e, and K_avcalculated as discussed previously.Custom Cmyk Printing Cake Gift Packing Box with Lid,Air Purification Activated Alumina. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine) (Reis et al.,1989;Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl.,1991;Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).,1991).Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)(1992), were lower, except for cystine and tyrosine,Resistan UV Grades PP Non Woven Fabric.

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CONCLUSIONS

New Scba Breathing Apparatus Equipment with Ce Certification. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Om4 Sc/Upc-LC/Upc Fiber Optic Cable) for the financial support.

REFERENCES

FRP Pultruded Pipe or GRP Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Night Vision Outdoor Surveillance CMOS Camera (BRC1930X)]

Brown, E. M; Thompson, C .J.,6090 CO2 Sealed Glass Cooling Laser Cutting Machine, M.M.(1994),Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. Analytical Biochemistry212,436-445[Painting Road Marking Vehicles Machine for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Fitness Equipment Swing (JMH-03). Biotechnology letters 14,6, 531-534[ Wire]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Handheld Conductivity Meter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle]

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable]

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ceramic Bearing Si3n4 608]

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, Part I.High Output Plastic Rubber Filing Masterbatch Extruder Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute]

High Precision Mold Manufacturer Plastic Injection Mould, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Wholesale Oxide Aluminium Black Sand Blast Media.Welded Stud Anchor Chain for Ship, 175-184[New Product CNC Machine Precision Auto Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Supply Coaxial Cable Rg 59 with Messenger]

Whitaker,J.R.(1963),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Analytical Chemistry35(12), 1950-1953[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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