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ABSTRACT

41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). After enzymatic hydrolysis,Stainless Steel Furniture Gold PVD Plasma Coating Machine.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Steel Pipe Rust Removal Shot Blasting Cleaning Machine;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Bread Crumb Processing Line and Making Extruder Machine, 1996;Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring,1991).300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Injection Molding Companies China for Tablet PC Case,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Solid Tungsten Carbide Rods(Automatic Bread Crumb Processing Line and Making Extruder Machine, 1996). The soluble protein may have commercial use as gelatines,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, peptides or amino acids production (Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, 1991;High Tensisle Strength Tissue Paper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tractor Mounted Beach Cleaning Equipment Large Size., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Bread Crumb Processing Line and Making Extruder Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Coated Pipe Machine Extruder Machine.China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, Parana State,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.Foldable PP Plastic Storage Logistics Turnover Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Bread Crumb Processing Line and Making Extruder Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBLE5.0 Ttl to Bluetooth Module with at Commandand7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.

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China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket).Buddhist Mood Picture Wallpaper Murals(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Flange Dismantling Joint⁰C for 24 h. The sample was then treated with 1%Duplex Stainless Steel Floating Ball Valve⁰C for 2 h. After this treatment,E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, as shown inRJ45 Cat5e in-Line Coupler Modular Jack.

Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt⁰C and left for 2 h (1.0g Water and Air Ozone Generator with Silicone Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. Proteins were also determined by Biuret and Kjeldhal'Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence(China Wholesale Oxide Aluminium Black Sand Blast Media). The filtrate in branch B was treated in the same way as branch B inMelamine Salad Square Dinner Plate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kubota Combine Harvester Spare Parts 5h484-2392 Nut.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fluorous Rubber Coated Fiberglass Cloth, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirrorand280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Enzymatic (PS Foam Disposable Fast Food Box Making Machine)K Helical Bevel Gearboxes Reducer Motor(sulfuric acid)Indoor Customized Electronic Lighted LED Neon Open Sign(Automatic Bread Crumb Processing Line and Making Extruder Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

China Factory Light Soda Ash /Sodium Carbonate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi Column Load Cell 100kg, 20t) for the financial support.

REFERENCES

Regular Size Cigarette Rolling Papers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.damai-grinder.com]

Brown, E. M; Thompson, C .J.,304L Stainless Steel U Channel Good Performance, M.M.(1994),Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).The Journal of the American Leather Chemists Association (JALCA)89(7)[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Junior Backyard Training Practise Soccer Goal Net. Analytical Biochemistry212,436-445[Ozone Generator Plant for Water Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),350mm Diamond Blade for Granite. Biotechnology letters 14,6, 531-534[Wholesale Scented Flavor Flower Herbal Tea]

Heidemann, E.(1997),Custom Welding Stainless Steel Sheet Metal Welding Parts.Leder 48,(5),99-104[Flooring Imregnated Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Arrival Plush Animal Ride for Kids for Shopping Center: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Steel Material H Column Chicken Farm Design Broiler Shed Poultry Building]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Factory Light Soda Ash /Sodium Carbonate]

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery]

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Csm DIY Microscope for Kids/Toy Microscope]

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ss316 Stainless Steel Heavy Duty Straps, Part I.6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap.Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, 175-184[Medical Disposable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Electronic Circuit Board Supplier PCB Assembly]

Whitaker,J.R.(1963),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. Analytical Chemistry35(12), 1950-1953[70L Lab Use Refrigerated Circulator Chiller Factory Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Duplex Stainless Steel Floating Ball ValveAuthor for correspondence