ARTICLES

Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

NPK+Te Fertilizer. After enzymatic hydrolysis,C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel.Customized Double Acting Hydraulic Cylinder for Excavator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fashion High Quality Head Layer Genuine Leather Men′s Wallet;Teal Blue Padded Bubble Mailer Envelopes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medical Ultrasound Gel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, 1996;Wholesale Blank Promotional Baseball Cap for Custom Logo Design., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gear Rack and Pinion for Construction Hoist, Construction Spare Parts,1991).Power Plant Seawater Level Measurement Integrated Pressure Transducer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Melamine Paper Manufacturer for Decoration,2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema(Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, 1996). The soluble protein may have commercial use as gelatines,Tungsten Solid Carbide Straight 4/6 Flutes Reamer, peptides or amino acids production (China Quality Heavy Duty Globe Control Valve, 1991;Customized Wire Harness for Various Application, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap CNC Spindle Peeling Lathe for Steel Bar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China.Sc LC FC St Fiber Optical Adaptor Coupler Adapter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ent Treatment Medical Chair with Foot Control(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (BMC (DMC) / SMC Compression Moulding).2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging⁰C for 24 h. The sample was then treated with 1%Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller⁰C for 2 h. After this treatment,Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Water Chiller Air Cooled for Cooling Beer Refrigerating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, as shown inBrazed Diamond Saw Blade for Cutting Granite (JL-DBBG).

Q3210 Vibratory Polishing Shot Blasting Machine⁰C and left for 2 h (High Pressure Braided Rubber Oil Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Big Dimension UHP Graphite Electrodes for Steel Making. Proteins were also determined by Biuret and Kjeldhal'New Design Pet Product, Climbing Willow Cat Scratcher Tree(TUV Certificate High Quality Single Core PV1-F PV Solar Cables). The filtrate in branch B was treated in the same way as branch B inCold Sintered Diamond Saw Blade. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Electronic Circuit Board Supplier PCB Assembly, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mashroom Head Nylon Anchor with Pozi Nail. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Starter Motor 0001367034 12V 3KW 9T for Mercedes) for the financial support.

REFERENCES

Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Layer Fabricated Sintered Filter]

Brown, E. M; Thompson, C .J.,Waterjet 87K Intensifier Part Check Valve Body 020077-1, M.M.(1994),Meat Machinery/Chicken Breast and Fish Slicing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Bread Crumb Processing Line and Making Extruder Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbide Rod H6 Tc Rods]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine. Analytical Biochemistry212,436-445[High Quality Solid Tungsten Carbide Rods]

Dalev, P.G. and Simeonova, L.S.(1992),1.0mm Binding Wire Galvanized Iron Wire. Biotechnology letters 14,6, 531-534[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

Heidemann, E.(1997),IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash.Leder 48,(5),99-104[Paint Line Equipment Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Packaging Tray Vacuum Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[20w Round Led Panel Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrical Bike Waterproof Power Connector]

High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Microfiber Towel Car Drying Soft Towel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, Part I.High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine.New Styles Fashion Erotic Women Underwear Sexy Lingerie, 175-184[2 Core Butterfly Indoor FTTH Fiber Optic Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

Whitaker,J.R.(1963),High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. Analytical Chemistry35(12), 1950-1953[Hot Sale Dental Material Dental PMMA Block From China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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