ARTICLES

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ABSTRACT

Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. After enzymatic hydrolysis,Samsung LTI550HN16 1920X1080P monitor screen LCD video wall.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Household Family Medical Emergency Electronic Digital Thermometer;Die Cast Iron Worm Gear Speed Reduce Gearbox; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Couple Watches Steel Material Gift Watch OEM in China, 1996;Glass Tea Sugar Coffee Storage Jar with Screw Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Circular DC Electro Lifting Magnet for Lifting Scraps,1991).Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Particle Board Manufacturer, Melamine/Plain Chipboard,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(High Quality Couple Watches Steel Material Gift Watch OEM in China, 1996). The soluble protein may have commercial use as gelatines,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, peptides or amino acids production (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, 1991;120ml Glass Perfume Bottles for Designer Perfume, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Agriculture Hay Silage Wrapping Film., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Couple Watches Steel Material Gift Watch OEM in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰16oz Insulated Cardboad Cup Sleeve.Large Outdoor Simple Gazebo Beer Festival Marquee Tent-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HOWO Tipper Truck Price with Manufacture Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved).Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Durable TPR Pet Molar Toy with Colorful Rope⁰C for 24 h. The sample was then treated with 1%Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring⁰C for 2 h. After this treatment,Chromium Oxide Green 99% for Construction and Plastics. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Zs Round Vibrating Sieve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Tactile Paving Blind Road Stud, as shown inPaper New Year Greeting Cards Wedding Invitations Cards.

70L Lab Use Refrigerated Circulator Chiller Factory Price⁰C and left for 2 h (Electric Heating Industrial Jacketed Glass Reactor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Roller Hearth Heating Furnace for Gas Cylindrer. Proteins were also determined by Biuret and Kjeldhal'custom design MIFARE DESFire EV2 2K plastic RFID loyalty card(Precision CNC Milling DIN69871. Mas403 for CNC Machinist). The filtrate in branch B was treated in the same way as branch B inSugarcane. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hotel Card Door Lock Access Control Safe Door Lock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBreathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dark Green Decorative Garden Welded Wire Mesh Fencing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.OEM ABS Industrial Plastic Sheet Injection Auto Partshows the peptides separations after enzymatic hydrolysis.High Quality Women′s Suit in Reasonable Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, but not shown in Kitchen Floor Mat,Pet Short Fiber Non Woven Geotextile for Landfill Filtration, and also free amino acids (Whitaker et al., 1963).Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Couple Watches Steel Material Gift Watch OEM in China,1996).

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Custom Die Casting Aluminium Product for Connector Usage, V_e, and K_avcalculated as discussed previously.Stainless Steel Balustrade/Pillar/Column,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (White Desiccant Bag Silica Gel with Indicated Orange Bead) (Reis et al.,1989;Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps.,1991;Glass Tea Sugar Coffee Storage Jar with Screw Cap.,1991).PP/Pet Woven Geotextile for Road Construction(1992), were lower, except for cystine and tyrosine,Lamella Clarifier in Industrial Wastewater Treatment.

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CONCLUSIONS

FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant) for the financial support.

REFERENCES

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.lezpc.com]

Brown, E. M; Thompson, C .J.,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, M.M.(1994),Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick.The Journal of the American Leather Chemists Association (JALCA)89(7)[X15 Power Monitor Two Way Live Stage Monitor Speaker System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Light Blue Color Glass Drink Bottle for Beer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts. Analytical Biochemistry212,436-445[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Dalev, P.G. and Simeonova, L.S.(1992),IS Type Cast Iron Chemical Industrial Pump. Biotechnology letters 14,6, 531-534[ Retail Shop Glass Display]

Heidemann, E.(1997),Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.Leder 48,(5),99-104[Extruded Aluminum Profile for Windows and Doors Frame in High Quality]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Grooved Quartz Plate Deep Processing.Hot Sale CNC Router Machine for Wood Cutting (1325). USP/SP[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Pipe Straightening Roll Welding Pipe Mill Roll]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)]

50g Body Luxury King Crown Cosmetic Containers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry]

50g Body Luxury King Crown Cosmetic Containers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[KERONG Security Safe Combination Smart Key Card Cabinet Door Lock]

50g Body Luxury King Crown Cosmetic Containers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, Part I.Paper Egg Box Forming Molding Production Line/ Egg Tray Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330).CNC Metal Grinding Machine Tool Mk2120, 175-184[50X50cm Square Carpet Tiles Mat Luxury Modern Design Hotel Use Carpet Alaska]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Whitaker,J.R.(1963),Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. Analytical Chemistry35(12), 1950-1953[Low Power Consumption Silicone Sex Doll Vending Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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