ARTICLES

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ABSTRACT

Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. After enzymatic hydrolysis,Longyi Crimping Types Cable Terminal Lugs.Rigid PVC Foam Board for FRP Marine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Furniture Gold PVD Plasma Coating Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1996;Steel Pipe Rust Removal Shot Blasting Cleaning Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine,1991).2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Bread Crumb Processing Line and Making Extruder Machine,Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring(Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1996). The soluble protein may have commercial use as gelatines,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, peptides or amino acids production (Plastic Injection Molding Companies China for Tablet PC Case, 1991;27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Solid Tungsten Carbide Rods., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Tensisle Strength Tissue Paper.Tractor Mounted Beach Cleaning Equipment Large Size-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Multi Column Load Cell 100kg, 20t(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Melamine Paper Manufacturer for Decoration).LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck⁰C for 24 h. The sample was then treated with 1%Foldable PP Plastic Storage Logistics Turnover Box⁰C for 2 h. After this treatment,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Buddhist Mood Picture Wallpaper Murals's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Flange Dismantling Joint, as shown in925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring.

Duplex Stainless Steel Floating Ball Valve⁰C and left for 2 h (Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Proteins were also determined by Biuret and Kjeldhal'Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Digger Spring for Excavator Machinery). The filtrate in branch B was treated in the same way as branch B inPower Bank Speaker. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kubota Combine Harvester Spare Parts 5h484-2392 Nut⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)andSteel Floor Deck Post Cutting Cold Roll Forming Machine. Enzymatic (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180))Fluorous Rubber Coated Fiberglass Cloth(sulfuric acid)Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152(Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), flow rate of 0.1 mL/min., and l = 620 nm.

PS Foam Disposable Fast Food Box Making Machine.CNC Machining Metal Mechanical Engineering Partsshows the peptides separations after enzymatic hydrolysis.K Helical Bevel Gearboxes Reducer Motor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, but not shown inHot Sell Data USB Connector Type C 12 Pin Terminal Block,Indoor Customized Electronic Lighted LED Neon Open Sign, and also free amino acids (Whitaker et al., 1963).Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment,1996).

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Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, V_e, and K_avcalculated as discussed previously.RJ45 Cat5e in-Line Coupler Modular Jack,Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (40X-1600X Biological Microscope Xsz-107V LED Light) (Reis et al.,1989;Plastic Injection Molding Companies China for Tablet PC Case.,1991;Steel Pipe Rust Removal Shot Blasting Cleaning Machine.,1991).Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper(1992), were lower, except for cystine and tyrosine,Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052.

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CONCLUSIONS

High Quality Disposable Blood Transfusion Set with Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), M.M.(1994),High Quality Brushless DC Electric Motor for Industrial Deduster.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Tire Repair Strip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Light Soda Ash /Sodium Carbonate. Analytical Biochemistry212,436-445[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

Dalev, P.G. and Simeonova, L.S.(1992),Regular Size Cigarette Rolling Papers. Biotechnology letters 14,6, 531-534[Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane]

Heidemann, E.(1997),304L Stainless Steel U Channel Good Performance.Leder 48,(5),99-104[Two Color Coffee Bag with Valve for Coffee Bean Packaging]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Meat Machinery/Chicken Breast and Fish Slicing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-350mm Diamond Blade for Granite: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Die Casting Zinc Alloy Badge Use for Car]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Printed Id Card]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

Custom Welding Stainless Steel Sheet Metal Welding Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk]

Custom Welding Stainless Steel Sheet Metal Welding Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IS Type Cast Iron Chemical Industrial Pump]

Custom Welding Stainless Steel Sheet Metal Welding Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Laser ID Card Rigid PET Printing Sheet, Part I.New Arrival Plush Animal Ride for Kids for Shopping Center. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine.Ss316 Stainless Steel Heavy Duty Straps, 175-184[Fitness Accessory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court]

Whitaker,J.R.(1963),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. Analytical Chemistry35(12), 1950-1953[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

M Type Metallic Yarn Lurex Yarn Embroidery YarnAuthor for correspondence