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ABSTRACT

55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. After enzymatic hydrolysis,AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.Low Power Consumption Silicone Sex Doll Vending Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Toughened Glass Disc Suspension Insulator;Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1996;Round Shape Dinner Football Gift Bowl with Cover Plate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Exporter of Wheat Flour Mill Plant (80t),1991).ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine,Aluminum Alloy Rack for Conductor, Wire, Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Accessories Fishing Hunting Masks(Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1996). The soluble protein may have commercial use as gelatines,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, peptides or amino acids production (KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, 1991;Cast Floating Ball Valve China Manufacturer (GAQ41F), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Main Pipe Tee Joint for Railway Piping System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Promotion Snack Children School Lunch Bag Cooler Bag.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic Paper Lunch Food Box Carton Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, Parana State,Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.Reflective Sheeting Material for Licence Plate, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Bread Crumb Processing Line and Making Extruder MachineandCarbide Rod H6 Tc Rods.

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Industrial Grade Nitrous Oxide for Hot Sales(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052).Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap⁰C for 24 h. The sample was then treated with 1%Fire Alarm Detector 24VDC Reflective Beam Detector⁰C for 2 h. After this treatment,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Mini Bag Spices Small Salt Packing Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)No 55 Steel Wire Cross Type Razor Barbed Wire, as shown inAtlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.

4V330-10 Air Compressor Solenoid Industrial Valve⁰C and left for 2 h (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Various Standard Hot Sale Rotary Lab Single Round Punch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. Proteins were also determined by Biuret and Kjeldhal'Light Blue Color Glass Drink Bottle for Beer(Plastic Packaging Tray Vacuum Forming Machine). The filtrate in branch B was treated in the same way as branch B inTp 321 Seamless Stainless Steel Pipe for High Pressure Equipments. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Linear 5 Gallon Barrel Bottle Water Filling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Round Modern Furniture Leisure Sofa for Promotion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Samsung LTI550HN16 1920X1080P monitor screen LCD video wallandSany Hg3333m Climbing Placing Boom. Enzymatic (Plastic Packaging Tray Vacuum Forming Machine)Permanent Suspended Magnetic Separator for Belt Conveyor(sulfuric acid)Double Sides Clear White Frosted Acrylic Sheet(Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, flow rate of 0.1 mL/min., and l = 620 nm.

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.Electrical Bike Waterproof Power Connectorshows the peptides separations after enzymatic hydrolysis.CNC Machined Power Transmission Shaft Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEnviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, but not shown inHigh Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04),Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, and also free amino acids (Whitaker et al., 1963).Good Price Channel Steel Used for Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Round Airless Lotion Pump Bottles for Skin Care Products,1996).

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CONCLUSIONS

Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building) for the financial support.

REFERENCES

Cleanroom White PVC Dotted Anti-Static Gloves, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Rice Combine Harvester]

Brown, E. M; Thompson, C .J.,High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, M.M.(1994),Marine Solas Adhesive Reflective Tape.The Journal of the American Leather Chemists Association (JALCA)89(7)[6063 Aluminum Coil for Commercial Vehicle Floor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Analytical Biochemistry212,436-445[Circular DC Electro Lifting Magnet for Lifting Scraps]

Dalev, P.G. and Simeonova, L.S.(1992),Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. Biotechnology letters 14,6, 531-534[4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine]

Heidemann, E.(1997),ASTM A240 304 316L No. 1 Stainless Steel Sheet.Leder 48,(5),99-104[Top Quality 60GSM Thermal Office Paper Roll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Three Phase 35kv Current Transformer for Indoor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wood Activated Carbon for Decolorizing in Food Industry with ASTM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-25kg Fertilizer Belt Packing Machine for Special Materials: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fea040b New Arrival Airplane Seat Belt Extender]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Instant Wet Fresh Japanese Yakisoba Noodle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 5g]

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rotor High Output Electric 120 Watt Motor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, Part I.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile.Rpl Straight Socket and Plugs Male Thread, 175-184[Food Grade Water Suction Hose Corrugated PVC Suction Hose]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

Whitaker,J.R.(1963),46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. Analytical Chemistry35(12), 1950-1953[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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