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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Canned Fruit Peach Processing Plant. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate

ABSTRACT

Garden Furniture Sofa Rattan Modular Corner Set with Cushions. After enzymatic hydrolysis,Stainless Steel Water Cream Bulk Storage Tank.Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1.0g Water and Air Ozone Generator with Silicone Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, 1996;16A/24A High Speed Wire Cable Braiding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot,1991).5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20 Inch 48V 750W Fat Tire Electric Bike with Double Seat,Agriculture Apple Orchard Reflective Pet Film,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)(Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, 1996). The soluble protein may have commercial use as gelatines,Small Boat Deck Knuckle Boom Ship Pedestal Crane, peptides or amino acids production (115mm Segmented Saw Blade for Concrete Cutting, 1991;Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Round Transparent Glass 750ml Liquor Glass Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Creative Design Metal Medal Promotion Gift of Sport.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ad-L13 Black Perfume Plastics Vial Bottle 10ml. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover.EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Whole Absorbtion Flexo Printing Soltting Die Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Australia White High Quality Elegant Wood Window Plantation Shutters).Factory Price 4X6 Acrylic Magnetic Photo Frame(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%200m Diesel Oil Hydraulic Water Well Drilling Machine⁰C for 24 h. The sample was then treated with 1%Custom Cmyk Printing Cake Gift Packing Box with Lid⁰C for 2 h. After this treatment,Air Purification Activated Alumina. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Resistan UV Grades PP Non Woven Fabric, as shown inChina Shipping Logistics to Stockholm.

New Scba Breathing Apparatus Equipment with Ce Certification⁰C and left for 2 h (Cleanroom White PVC Dotted Anti-Static Gloves). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.FRP Pultruded Pipe or GRP Profile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6090 CO2 Sealed Glass Cooling Laser Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator(Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface). The filtrate in branch B was treated in the same way as branch B inAluminum Head Suction Cup Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Fitness Equipment Swing (JMH-03). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSheet Metal Distribution Cabinet with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Welded Stud Anchor Chain for Ship.Fashion High Quality Head Layer Genuine Leather Men′s Walletshows the peptides separations after enzymatic hydrolysis.SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLinear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, but not shown inUniversal Mirco Forklift Car Bus Truck Seats Switch Sensors,Line Scan Uvss System Portable Under Vehicle Surveillance, and also free amino acids (Whitaker et al., 1963).Folding Steel Manual Wheelchair with Chrome Frame. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine,1996).

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CONCLUSIONS

Wrinkle Effect Crease UV Drying Machine UV Curing System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mexico Single Expansion Anchor with YZP) for the financial support.

REFERENCES

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Manufacturer DC and Cc Lamps/Cookware Aluminium Circle]

Brown, E. M; Thompson, C .J.,Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, M.M.(1994),20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tefude Feeding and Packaging Line for Food with Tray. Analytical Biochemistry212,436-445[3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts. Biotechnology letters 14,6, 531-534[Waw 8 Ton Logistics Light Truck for Transportation]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sheet Metal Distribution Cabinet with High Quality]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Artnet Node Artnet to DMX Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polyester Square Net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Custom Sheet Metal Fabrication]

Felt Disc Polishing Sanding Disc with Wool Felt Diam, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Boat Deck Knuckle Boom Ship Pedestal Crane]

Felt Disc Polishing Sanding Disc with Wool Felt Diam,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Double Sides Clear White Frosted Acrylic Sheet]

Felt Disc Polishing Sanding Disc with Wool Felt Diam, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, Part I.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

240T goodeng water/oil/gas pipe drilling rig HDD machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 175-184[120pcs Solder Seal Wire Connectors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Whitaker,J.R.(1963),Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Analytical Chemistry35(12), 1950-1953[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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