ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Teal Blue Padded Bubble Mailer Envelopes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Medical Ultrasound Gel

ABSTRACT

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Key words;Power Plant Seawater Level Measurement Integrated Pressure Transducer;High Quality Melamine Paper Manufacturer for Decoration; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 1996;Tungsten Solid Carbide Straight 4/6 Flutes Reamer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Quality Heavy Duty Globe Control Valve,1991).Customized Wire Harness for Various Application. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap CNC Spindle Peeling Lathe for Steel Bar,Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a(Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 1996). The soluble protein may have commercial use as gelatines,6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, peptides or amino acids production (Sc LC FC St Fiber Optical Adaptor Coupler Adapter, 1991;Ent Treatment Medical Chair with Foot Control, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Leaf Shape Pearl Jewelry Wedding Tiara.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Starter Motor 0001367034 12V 3KW 9T for Mercedes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte.HPMC Hydroxy Propyl Methyl Cellulose Additives-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop, Parana State,Medical Ultrasound Gel.Water Chiller Air Cooled for Cooling Beer Refrigerating, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBeauty Salon Equipment Anti Wrinkle Removal CO2 Laser MachineandReusable PLA and Pbat Tea Packing Pouch Supplier in China.

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High Quality Cheap Price Disposable IV Infusion Set with Precise Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Viton Mechanical Rubber Oil Seal).Q3210 Vibratory Polishing Shot Blasting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear⁰C for 24 h. The sample was then treated with 1%Big Dimension UHP Graphite Electrodes for Steel Making⁰C for 2 h. After this treatment,New Design Pet Product, Climbing Willow Cat Scratcher Tree. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate, as shown inChia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Femo Ferro Molybdenum Powder Lump 55/60. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Painting Road Marking Vehicles Machine for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automotive Rubber Parts for Any Rubber Material, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Die Casting Zinc Alloy Badge Use for Car, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Delicate Handmade Round Natural Stone Chain NecklaceandHair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge. Enzymatic (CREE Chip 24W 28W 30W LED Downlight)Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove(sulfuric acid)Three Phase 35kv Current Transformer for Indoor(Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Shipping Logistics to Stockholm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Shipping Logistics to Stockholm, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Lathe Main Parts with Good Quality.XL-21 Motive Electrical Switchgear Power Cabinetshows the peptides separations after enzymatic hydrolysis.Good Price High Torque 12mm Low Speed Small Geared Motor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, but not shown inFelt Disc Polishing Sanding Disc with Wool Felt Diam,Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card, and also free amino acids (Whitaker et al., 1963).Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema,1996).

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CONCLUSIONS

IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging) for the financial support.

REFERENCES

Wholesales Stackable Coffee Iron Chair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Circle 1060]

Brown, E. M; Thompson, C .J.,OEM Eye Anti-Aging Serum, M.M.(1994),Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. Analytical Biochemistry212,436-445[Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button]

Dalev, P.G. and Simeonova, L.S.(1992),Microfiber Towel Car Drying Soft Towel. Biotechnology letters 14,6, 531-534[ Rapid]

Heidemann, E.(1997),Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.Leder 48,(5),99-104[Factory Supply Square Shape Glass Honey Jar with Black Metal Lid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

Fashion High Quality Head Layer Genuine Leather Men′s Wallet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring.STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food. USP/SP[Luxury Hotel Furniture Antique Design Marble Side Table]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[BMC (DMC) / SMC Compression Moulding]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Low Price Sweep Frequency Response Analyzer for Power Transformer Test]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pressure Braided Rubber Oil Hose]

New Styles Fashion Erotic Women Underwear Sexy Lingerie, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

New Styles Fashion Erotic Women Underwear Sexy Lingerie,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Unique Handle 24mm Water Bottle Caps]

New Styles Fashion Erotic Women Underwear Sexy Lingerie, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, Part I.200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light]

Fully Automatic Water Bottle Lids Labeling Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels.New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera, 175-184[www.chenanjixie.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Construction in Poultry House with Good Quality and Installation]

Whitaker,J.R.(1963),Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs. Analytical Chemistry35(12), 1950-1953[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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