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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

X15 Power Monitor Two Way Live Stage Monitor Speaker System. After enzymatic hydrolysis,Australia White High Quality Elegant Wood Window Plantation Shutters.Big Smart Hydroponic Planter Indoor Garden Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion;Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling Custom Sheet Metal Fabrication, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, 1996;Powder Coating Paint Lines Systems for Factory /Plants., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC,1991).Mini Pencil Tag with Lanyard (YS211). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100% Latex Free Endotracheal Tube with PVC Material,Neodymium Magnet Pot Assembly with Strong Weight,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System(15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer of Venlo PC or Venlo Glass Greenhouse, peptides or amino acids production (Artnet Node Artnet to DMX Controller, 1991;6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CH Series Pid Intelligent Temperature Controller CH102.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Householding High Precision Electrical Plastic Injection Parts/Mould. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Women Hair Accessories Hair Bow Headband for Girls.Round Hole Carbon Steel Pipe Perforated for Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Manufacturer Stainless Steel Floor Grating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Fitness Equipment Swing (JMH-03)).SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Rubber Pressed Mixing Machine with Ce Certification⁰C for 24 h. The sample was then treated with 1%Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600⁰C for 2 h. After this treatment,4K Giant Whiteboard Interactive Panel for University Whiteboard. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), as shown in4K Giant Whiteboard Interactive Panel for University Whiteboard.

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting⁰C and left for 2 h (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Practical Edible Silicone Kitchenware (YB-ty-29). Proteins were also determined by Biuret and Kjeldhal'4 Inch Quarter Round PCD Diamond Grinding Cup Wheel(100kw Cummins Diesel Generator Set). The filtrate in branch B was treated in the same way as branch B inAirless Spray Pump Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Cube Ice Maker Parts / Ice Machines Maker Cube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips) for the financial support.

REFERENCES

5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rebar Tie Wire Tw1061t Fit Automatic Binding Machine Rb441t]

Brown, E. M; Thompson, C .J.,20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, M.M.(1994),Agriculture Apple Orchard Reflective Pet Film.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Beans]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). Analytical Biochemistry212,436-445[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Dalev, P.G. and Simeonova, L.S.(1992),Small Boat Deck Knuckle Boom Ship Pedestal Crane. Biotechnology letters 14,6, 531-534[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Heidemann, E.(1997),115mm Segmented Saw Blade for Concrete Cutting.Leder 48,(5),99-104[Seawater Flake Ice Evaporator with Electric Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit.China Round Transparent Glass 750ml Liquor Glass Bottle. USP/SP[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Produce Medical Machining Custom Metal Milling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Creative Design Metal Medal Promotion Gift of Sport: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Machined Spare Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Micro Button Type Load Cell Compression Load Cell 10kg]

Ad-L13 Black Perfume Plastics Vial Bottle 10ml, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lifting Thread with Needle for Plastic Surgery]

Ad-L13 Black Perfume Plastics Vial Bottle 10ml,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Ad-L13 Black Perfume Plastics Vial Bottle 10ml, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), Part I.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[BLE5.0 Ttl to Bluetooth Module with at Command]

Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.Mexico Single Expansion Anchor with YZP, 175-184[Anchor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Whitaker,J.R.(1963),Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. Analytical Chemistry35(12), 1950-1953[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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