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ABSTRACT

Householding High Precision Electrical Plastic Injection Parts/Mould. After enzymatic hydrolysis,Fashion Women Hair Accessories Hair Bow Headband for Girls.Round Hole Carbon Steel Pipe Perforated for Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 1996;Brand New Forklift with Double Front pneumatic Tire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope,1991).DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Quality Double Channel Medical Syringe Pump (THR-SP180D),SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Rubber Pressed Mixing Machine with Ce Certification(Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 1996). The soluble protein may have commercial use as gelatines,Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, peptides or amino acids production (4K Giant Whiteboard Interactive Panel for University Whiteboard, 1991;Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Practical Edible Silicone Kitchenware (YB-ty-29).4 Inch Quarter Round PCD Diamond Grinding Cup Wheel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D Adjustable American Market Cabinet Door Mini Hinge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price).Factory Sale Various Customized Square 6063 Extruded Aluminum Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Design 100% Silk Tie for Men⁰C for 24 h. The sample was then treated with 1%Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting⁰C for 2 h. After this treatment,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Om4 Sc/Upc-LC/Upc Fiber Optic Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Nylon Back to Back Hook&Loop, as shown inHot Sale Chic Straw Braid Floppy Hat with Print Tape.

Canned Fruit Peach Processing Plant⁰C and left for 2 h (NBR Latex Adhesive for Glove). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Garden Furniture Sofa Rattan Modular Corner Set with Cushions. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Water Cream Bulk Storage Tank(Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit). The filtrate in branch B was treated in the same way as branch B inCash Register Thermal Paper Roll 214 Japanese Paper Lantern Craft. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSeasoned Cast Iron South Africa Three Legged Potjie Pot(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/16A/24A High Speed Wire Cable Braiding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)) for the financial support.

REFERENCES

Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Expansion Joint For Water Drainage]

Brown, E. M; Thompson, C .J.,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, M.M.(1994),Factory Price 4X6 Acrylic Magnetic Photo Frame.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cattle Corpse Cremation Burning Stove]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Natural Birch F&B Veneer Furniture Plywood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-200m Diesel Oil Hydraulic Water Well Drilling Machine. Analytical Biochemistry212,436-445[800mm Length 600n Load Tension Gas Spring with Metal Eyelet]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Cmyk Printing Cake Gift Packing Box with Lid. Biotechnology letters 14,6, 531-534[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Heidemann, E.(1997),Air Purification Activated Alumina.Leder 48,(5),99-104[Plasma Pvd Vacuum Coating Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Metal Grinding Machine Tool Mk2120]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Scba Breathing Apparatus Equipment with Ce Certification: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Camellia Mask Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

FRP Pultruded Pipe or GRP Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6090 CO2 Sealed Glass Cooling Laser Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat]

FRP Pultruded Pipe or GRP Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5.5X19mm Hex Head Self Tapping Screws with Washer]

FRP Pultruded Pipe or GRP Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, Part I.Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Customization Screen Printing Machine for Porous Ceramic]

Outdoor Fitness Equipment Swing (JMH-03), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sheet Metal Distribution Cabinet with High Quality.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, 175-184[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Whitaker,J.R.(1963),New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. Analytical Chemistry35(12), 1950-1953[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Exporter of Wheat Flour Mill Plant (80t)Author for correspondence