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ABSTRACT

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. After enzymatic hydrolysis,Waterproof PVDF Aluminum Curtain Wall Panel.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sany Hg3333m Climbing Placing Boom, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Packaging Tray Vacuum Forming Machine, 1996;Permanent Suspended Magnetic Separator for Belt Conveyor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Sides Clear White Frosted Acrylic Sheet,1991).5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty,CNC Machined Power Transmission Shaft Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(Plastic Packaging Tray Vacuum Forming Machine, 1996). The soluble protein may have commercial use as gelatines,Good Price Channel Steel Used for Machinery, peptides or amino acids production (Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, 1991;Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Direct Sale Patent up to Down Rubber Tile Curing Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Packaging Tray Vacuum Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Real Leather Wallet Mens Designer Leather Wallets.Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Port Small Automatic Electronic Plumbing Ball Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, Parana State,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Packaging Tray Vacuum Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theA5 PU Cover Loose Leaf Business Diary Book Notepad NotebookandMedical Products PTC Puncture Needle Epidural Sharp Capability Fexible.

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ASTM A240 304 316L No. 1 Stainless Steel Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Hot Rolled Steel U Channel China Supplier).12V 24V 220V Digital Temperature Controller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price⁰C for 24 h. The sample was then treated with 1%25kg Fertilizer Belt Packing Machine for Special Materials⁰C for 2 h. After this treatment,Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rotor High Output Electric 120 Watt Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, as shown inPowder Coating Paint Lines Systems for Factory /Plants.

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed⁰C and left for 2 h (Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. Proteins were also determined by Biuret and Kjeldhal'Rpl Straight Socket and Plugs Male Thread(20 Inch 48V 750W Fat Tire Electric Bike with Double Seat). The filtrate in branch B was treated in the same way as branch B inCorn Tortilla Doritos Chips Manufacturing Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Metal Stamping Stainless Steel Parts with CNC Machining. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSs Wafer Type Check Valve Dn32 Dn40 Dn50(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/WPC Wood Plastic Board Equipment Extruder Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Metal Stamping Welding Spare Parts for Farming.Paper New Year Greeting Cards Wedding Invitations Cardsshows the peptides separations after enzymatic hydrolysis.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Heating Industrial Jacketed Glass Reactor, but not shown inPrecision CNC Milling DIN69871. Mas403 for CNC Machinist,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, and also free amino acids (Whitaker et al., 1963).275ml Frosted Clear Glass Drinking Bottle for Juice. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Packaging Tray Vacuum Forming Machine,1996).

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Factory Direct Sales Kids Boy Cotton Children T Shirt, V_e, and K_avcalculated as discussed previously.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener,15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Blank Promotional Baseball Cap for Custom Logo Design) (Reis et al.,1989;Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.,1991;Permanent Suspended Magnetic Separator for Belt Conveyor.,1991).Light Blue PVC Plastic Sheet and PVC Film(1992), were lower, except for cystine and tyrosine,Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy.

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CONCLUSIONS

Metric Female Cone Hydraulic Hose Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail) for the financial support.

REFERENCES

Hot Sale Dental Material Dental PMMA Block From China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.tysonleather.com]

Brown, E. M; Thompson, C .J.,Plastic Waste Pet Flakes Recycling Washing Machine, M.M.(1994),Deposited Stainless Steel Rivet Nut with Knurled.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Wrap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White Desiccant Bag Silica Gel with Indicated Orange Bead]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Stainless Steel Mechanical Filter Hosing. Analytical Biochemistry212,436-445[Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper]

Dalev, P.G. and Simeonova, L.S.(1992),Paper New Year Greeting Cards Wedding Invitations Cards. Biotechnology letters 14,6, 531-534[Silicon Carbide Foundry Porous Ceramic Foam Filter]

Heidemann, E.(1997),Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).Leder 48,(5),99-104[AC Voltage Automatic Stabilizer for Brushless Generator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[X15 Power Monitor Two Way Live Stage Monitor Speaker System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Customization Screen Printing Machine for Porous Ceramic: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Retail Shop Glass Display]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alloy Rims Wheels]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Fea040b New Arrival Airplane Seat Belt Extender, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

Fea040b New Arrival Airplane Seat Belt Extender,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)]

Fea040b New Arrival Airplane Seat Belt Extender, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Square Shape Glass Candle Holder for One Piece Tealight Candle, Part I.1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH]

Medical Autoclavable Surgical Thoracoscopy Trocar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.Easy Foldable Kids Walking Chair Toys Baby Walker, 175-184[Surface Mounted LED Light Channel Square Aluminum Profile for Indoor LED Strip Cabinet Lighting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)]

Whitaker,J.R.(1963),Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. Analytical Chemistry35(12), 1950-1953[Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

KERONG Security Safe Combination Smart Key Card Cabinet Door LockAuthor for correspondence