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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tct Circular Saw Blade &Diamond Saw Blade, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable, 1996;Lifting Thread with Needle for Plastic Surgery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls,1991).LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fudan Compatible Card 1k RFID Card 13.56MHz,Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Biodegradable Food Packaging Take out Food Boxes(Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable, 1996). The soluble protein may have commercial use as gelatines,Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, peptides or amino acids production (Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, 1991;AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Ton Small Vertical Plastic Injection Molding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White Hand Carved Angel Sculpture, Marble Child Statue for Garden.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foska Mini Size High Quality Heavy Duty Stapler. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5.5X19mm Hex Head Self Tapping Screws with Washer.Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stone Carving Flower Pot for Garden Decoration).Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5 Star European Antique Hotel Furniture⁰C for 24 h. The sample was then treated with 1%4.0MP Ahd Tvi CVBS HD CCTV Camera Module⁰C for 2 h. After this treatment,Active Demand Plastic 65 Liter Laundry Basket Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Organic Fertilizer Compost Turning Machine, Compost Turner Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Insulation Top Hung Aluminum Awning Window with Mosquito Net, as shown inAnimal Carcass Incinerator Hospital Garbage Incinerator.

4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger⁰C and left for 2 h (Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sell Data USB Connector Type C 12 Pin Terminal Block. Proteins were also determined by Biuret and Kjeldhal'Unique Handle 24mm Water Bottle Caps(Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale). The filtrate in branch B was treated in the same way as branch B inTuya Wifi Switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Band Cable Ties Wire Strap Steel Banding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Acid Water Pulse Output Liquid Orifice Type Flow Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2019 New Water Quality TDS Test.Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lightsshows the peptides separations after enzymatic hydrolysis.Top Furniture Hotel Dining Furniture Metal Hotel Table Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, but not shown inA5 PU Cover Loose Leaf Business Diary Book Notepad Notebook,Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital, and also free amino acids (Whitaker et al., 1963).Machinery Parts Aeroplane Engine CNC Milling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable,1996).

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CONCLUSIONS

Portable Powerful Charger with Dual Pd Charger and Type-C Charger. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD) for the financial support.

REFERENCES

Flexible Vj Rigid Shaft Coupling for Pipe Flange, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cooker Pcb Board]

Brown, E. M; Thompson, C .J.,Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting, M.M.(1994),Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Heating Industrial Jacketed Glass Reactor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers. Analytical Biochemistry212,436-445[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry. Biotechnology letters 14,6, 531-534[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Heidemann, E.(1997),100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price.Leder 48,(5),99-104[125tons Hydraulic Press Brake 4 Meter Bending Machine with Safety Protection]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3-35% Paddy Moisture Meter/ Corn Moisture Meter/Grain Digital Moisture Meter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

2017 China Hot Sale Water Screen Movie Fountain, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

2017 China Hot Sale Water Screen Movie Fountain,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Wrap]

2017 China Hot Sale Water Screen Movie Fountain, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, Part I.LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Desiccant Bag Silica Gel with Indicated Orange Bead]

Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale.Latest New Hho Engine Oil Carbon Remover, 175-184[Soft Galvanized Steel Coil/Strip Gi/Gl for PPGI PPGL0.13-2.6]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper]

Whitaker,J.R.(1963),Wood Chips Rotary Dryer Sawdust Pellet Drying Machine. Analytical Chemistry35(12), 1950-1953[Silicon Carbide Foundry Porous Ceramic Foam Filter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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