ARTICLES

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ABSTRACT

Organic Fertilizer Compost Turning Machine, Compost Turner Machine. After enzymatic hydrolysis,Insulation Top Hung Aluminum Awning Window with Mosquito Net.4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Unique Handle 24mm Water Bottle Caps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Band Cable Ties Wire Strap Steel Banding, 1996;Acid Water Pulse Output Liquid Orifice Type Flow Meter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware,1991).Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay,Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production(Stainless Steel Band Cable Ties Wire Strap Steel Banding, 1996). The soluble protein may have commercial use as gelatines,Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen, peptides or amino acids production (High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, 1991;Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Door Heated Holding Carts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 New Water Quality TDS Test. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Band Cable Ties Wire Strap Steel Banding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Top Furniture Hotel Dining Furniture Metal Hotel Table Chair.Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Machinery Parts Aeroplane Engine CNC Milling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Painted G80 Cargo Lashing Chain with Hooks, Parana State,Active Demand Plastic 65 Liter Laundry Basket Mould.Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Band Cable Ties Wire Strap Steel Banding, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePolyester Hunting Clothes Breathable Sniper Army Airsoft UniformandDeposited Stainless Steel Rivet Nut with Knurled.

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Automatic Feeding Customized Multi Layers Shirts Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester).Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Portable Powerful Charger with Dual Pd Charger and Type-C Charger⁰C for 24 h. The sample was then treated with 1%Flexible Vj Rigid Shaft Coupling for Pipe Flange⁰C for 2 h. After this treatment,Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers, as shown inAuto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2017 China Hot Sale Water Screen Movie Fountain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuper Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , pH 3.25., mobile phase B: buffer solution of 0.01 mol/Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Waterproof PVDF Aluminum Curtain Wall PanelandLatest New Hho Engine Oil Carbon Remover. Enzymatic (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine)Delicate Handmade Round Natural Stone Chain Necklace(sulfuric acid)Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price(Stainless Steel Band Cable Ties Wire Strap Steel Banding, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, flow rate of 0.1 mL/min., and l = 620 nm.

Embossed Aluminium/ Aluminum Household Foil 8011-O.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverageshows the peptides separations after enzymatic hydrolysis.High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, but not shown inNew Styles Fashion Erotic Women Underwear Sexy Lingerie,Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, and also free amino acids (Whitaker et al., 1963).Cross Hex Flange Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Band Cable Ties Wire Strap Steel Banding,1996).

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Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Priceand115mm Segmented Saw Blade for Concrete Cuttingshow the gel chromatographic separation of soluble protein with Sephadex G-Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.Full Color Indoor Outdoor LED Curtain TV Display, and another peak at 45 mL not shown inOEM Factory New Girls Nuts Crunch Cheap Price, for peptides lower than 3000 Da.High Quality Plastic Injection Mold Parts Square Interlocks Maker(Condell et al.,1993) .

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LED Outdoor Solar Lawn Garden Lighting for Wall, V_e, and K_avcalculated as discussed previously.3-Pin Australia Power Cord Plug with SAA,Sludge Oil Separation Centrifuge. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine) (Reis et al.,1989;High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing.,1991;Acid Water Pulse Output Liquid Orifice Type Flow Meter.,1991).8011 Aluminum Foil for Cap Used(1992), were lower, except for cystine and tyrosine,Pepper Peanut Sauce Colliod Miller Grinding Machine.

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CONCLUSIONS

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electrical Bike Waterproof Power Connector) for the financial support.

REFERENCES

12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Foil Vacuum Packing Machine]

Brown, E. M; Thompson, C .J.,Electronic Foldable for Patient Treatment Ent Unit Chair, M.M.(1994),Plastic Pushbutton Ultrasonic Spin Welding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. Analytical Biochemistry212,436-445[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

Dalev, P.G. and Simeonova, L.S.(1992),Double Shaft Paper Cardboard Waste Plastic Shredder Machine. Biotechnology letters 14,6, 531-534[Polypropylene Strapping Band Production Line/PP Strap Making Machine]

Heidemann, E.(1997),Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.Leder 48,(5),99-104[2000mm Thin Blade Slitter Scorer Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

5 Star European Antique Hotel Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Desktop Single Wave Soldering Machine/Wave Soldering Machine.Manufacturers White Dinnerware Set Fast Food Tray Bento Box. USP/SP[Furniture Grade E1 Glue Plywood with Competitive Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Price Graphite Electrode HP 300mm UHP: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.jiaguanadulttoys.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wld8200 Auto Spray Paint Booth-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo]

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated]

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), Part I.Animal Carcass Incinerator Hospital Garbage Incinerator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Colorful Braided Fabric Cable Round]

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Density Crust Foam Board/Celuka Board.Mango Pulp Juice Processing Production Line, 175-184[Ppr Injection Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant]

Whitaker,J.R.(1963),Laser Cut Sheet Metal Fiber Laser Cutting Machine. Analytical Chemistry35(12), 1950-1953[Rotor High Output Electric 120 Watt Motor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

5 Star European Antique Hotel FurnitureAuthor for correspondence