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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-FRP Pultruded Pipe or GRP Profile. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,6090 CO2 Sealed Glass Cooling Laser Cutting Machine

ABSTRACT

Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. After enzymatic hydrolysis,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.Outdoor Fitness Equipment Swing (JMH-03). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, 1996;Metal Animal Longhorn Skull Pendant Longhorn Skull Charm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant,1991).Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Output Plastic Rubber Filing Masterbatch Extruder Machine,High Precision Mold Manufacturer Plastic Injection Mould,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Wholesale Oxide Aluminium Black Sand Blast Media(Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, 1996). The soluble protein may have commercial use as gelatines,Welded Stud Anchor Chain for Ship, peptides or amino acids production (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1991;Line Scan Uvss System Portable Under Vehicle Surveillance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Folding Steel Manual Wheelchair with Chrome Frame., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Style Lady Alloy Quartz Watch with Simple Dial. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Building Decoration Material Acrylic Dispersant for Coating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, Parana State,6090 CO2 Sealed Glass Cooling Laser Cutting Machine.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw ExtruderandCREE Chip 24W 28W 30W LED Downlight.

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20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable).Tefude Feeding and Packaging Line for Food with Tray(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts⁰C for 24 h. The sample was then treated with 1%Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine⁰C for 2 h. After this treatment,CMP No Nc Metal Switch Black 25mm Illuminated Push Button. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, as shown inCustomized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components.

Felt Disc Polishing Sanding Disc with Wool Felt Diam⁰C and left for 2 h (Stainless Steel Handrail Bar Holder for 16mm Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. Proteins were also determined by Biuret and Kjeldhal'100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth(6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China). The filtrate in branch B was treated in the same way as branch B inwww.searchlight-henlin.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,240T goodeng water/oil/gas pipe drilling rig HDD machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Colorful Braided Fabric Cable Round⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Universal Wholesale Hot Sale Custom Blank Keys, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PVC Powder Coated Galvanized Metal Welded Wire Mesh FenceandRotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. Enzymatic (Waw 8 Ton Logistics Light Truck for Transportation)Hot Forging Lathe Spindle Forged Spindle(sulfuric acid)Hot Sale Chic Straw Braid Floppy Hat with Print Tape(Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, flow rate of 0.1 mL/min., and l = 620 nm.

Lamella Clarifier in Sewage Tretament Plant for Water Purification.Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fenceshows the peptides separations after enzymatic hydrolysis.BMC (DMC) / SMC Compression Moulding(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, but not shown inOutdoor Multifunctional Solar Power Flashlight (SYSG-517),Ring Caps &Plastic Tube Pop Novelties, and also free amino acids (Whitaker et al., 1963).Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting,1996).

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CONCLUSIONS

Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Supply Straight Ball Joint Bearing 02b04) for the financial support.

REFERENCES

OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial Fans For Sale]

Brown, E. M; Thompson, C .J.,Handmade Natural Bamboo Basket / Bamboo Flower Pot, M.M.(1994),Stainless Steel Handrail Bar Holder for 16mm Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[St Fiber Optic Adaptor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shac Flange Linear Bearing. Analytical Biochemistry212,436-445[High Quality Melamine Paper Manufacturer for Decoration]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Biotechnology letters 14,6, 531-534[925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machining Metal Mechanical Engineering Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale CAS 24916-51-6 Acetylspiramycin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable]

Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Hot Rolled Steel U Channel China Supplier-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[50g Body Luxury King Crown Cosmetic Containers]

Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:M Type Metallic Yarn Lurex Yarn Embroidery Yarn, Part I.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards]

Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, 175-184[Replacement for Sony Xperia Z3 LCD L55t D6603 D6653 Original Touch Screen Display Digitizer Assembly for Sony Z3 LCD AAA]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21]

Whitaker,J.R.(1963),Anti-Pollution Modified Acrylic Solid Surface for Countertops. Analytical Chemistry35(12), 1950-1953[High Precision Mold Manufacturer Plastic Injection Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

WPC Wood Plastic Board Equipment Extruder MachineAuthor for correspondence