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ABSTRACT

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle. After enzymatic hydrolysis,Durable TPR Pet Molar Toy with Colorful Rope.Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chromium Oxide Green 99% for Construction and Plastics;Zs Round Vibrating Sieve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Tactile Paving Blind Road Stud, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (70L Lab Use Refrigerated Circulator Chiller Factory Price, 1996;Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Roller Hearth Heating Furnace for Gas Cylindrer,1991).custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016,Hotel Card Door Lock Access Control Safe Door Lock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer(70L Lab Use Refrigerated Circulator Chiller Factory Price, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel DIN7981 Pan Head Self Tapping Screw, peptides or amino acids production (19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, 1991;Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dark Green Decorative Garden Welded Wire Mesh Fencing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Csm DIY Microscope for Kids/Toy Microscope.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Oak Veneer Laminated Plywood for Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (70L Lab Use Refrigerated Circulator Chiller Factory Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib.Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed HDMI Cable Wholesale HDMI with Ethernet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Machinery Parts Aeroplane Engine CNC Milling).Custom Die Casting Aluminium Product for Connector Usage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Balustrade/Pillar/Column⁰C for 24 h. The sample was then treated with 1%Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller⁰C for 2 h. After this treatment,PP/Pet Woven Geotextile for Road Construction. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lamella Clarifier in Industrial Wastewater Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, as shown inFactory Direct Sale Patent up to Down Rubber Tile Curing Press.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Grooved Quartz Plate Deep Processing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/50g Body Luxury King Crown Cosmetic Containers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet Short Fiber Non Woven Geotextile for Landfill Filtration) for the financial support.

REFERENCES

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Clinical Mercury Thermometer at Competitive Price]

Brown, E. M; Thompson, C .J.,Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, M.M.(1994),Minimalist Slim Real Leather Front Pocket Card Wallet.The Journal of the American Leather Chemists Association (JALCA)89(7)[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Natural Cleaning Konjak Sponge. Analytical Biochemistry212,436-445[100kw Cummins Diesel Generator Set]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor Load Break Switch Isolating Switch. Biotechnology letters 14,6, 531-534[Easy Foldable Kids Walking Chair Toys Baby Walker]

Heidemann, E.(1997),Biodegradable Disposible Straw Pulp Food Box Ppp-1007.Leder 48,(5),99-104[Tableware Dinner Ware Matt Surface Green Bamboo Durable Safe Plastic Melamine Distinctive Rectangle/Rectangular Tray Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Rubber Pressed Mixing Machine with Ce Certification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-NBR Latex Adhesive for Glove: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.fashiongusta.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Cheap Wooden House Zinc Plated Angle Bracket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

Cheap Wooden House Zinc Plated Angle Bracket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Latex Free Endotracheal Tube with PVC Material]

Cheap Wooden House Zinc Plated Angle Bracket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, Part I.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solar Powered Outdoor Lighting LED Solar Wall Light]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Whitaker,J.R.(1963),12 Inch King Mattress Latex Foam Memory Foam Mattress. Analytical Chemistry35(12), 1950-1953[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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