ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Gradient Print Overlay Push Button Membrane Keypad Switch. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X

ABSTRACT

Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). After enzymatic hydrolysis,Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle;Low Power Consumption Silicone Sex Doll Vending Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toughened Glass Disc Suspension Insulator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1996;63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Round Airless Lotion Pump Bottles for Skin Care Products,1991).Round Shape Dinner Football Gift Bowl with Cover Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Exporter of Wheat Flour Mill Plant (80t),ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine(Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1996). The soluble protein may have commercial use as gelatines,Aluminum Alloy Rack for Conductor, Wire, Cable, peptides or amino acids production (Outdoor Accessories Fishing Hunting Masks, 1991;Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;KERONG Security Safe Combination Smart Key Card Cabinet Door Lock., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cast Floating Ball Valve China Manufacturer (GAQ41F).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Main Pipe Tee Joint for Railway Piping System.Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Promotion Snack Children School Lunch Bag Cooler Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Toughened Glass Insulator for Transmission Lines/Glass Insulator U160bAlcalase2.5 L DX,Full Automatic Paper Lunch Food Box Carton Forming Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Single Core Solid Copper Conductor Rg Coaxial Cable); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building, Parana State,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWood Fireplace Double Skin Chimney Flue Pipesand12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.

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P180

Life Size 170cm Silicone Sex Dolls Real Love Doll for Male(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide).Reflective Sheeting Material for Licence Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Grade Nitrous Oxide for Hot Sales⁰C for 24 h. The sample was then treated with 1%Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove⁰C for 2 h. After this treatment,Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fire Alarm Detector 24VDC Reflective Beam Detector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, as shown inMen Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear.

Automatic Mini Bag Spices Small Salt Packing Packaging Machine⁰C and left for 2 h (Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.No 55 Steel Wire Cross Type Razor Barbed Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4V330-10 Air Compressor Solenoid Industrial Valve. Proteins were also determined by Biuret and Kjeldhal'Professional Various Standard Hot Sale Rotary Lab Single Round Punch(Agriculture Apple Orchard Reflective Pet Film). The filtrate in branch B was treated in the same way as branch B inm.yongchengnature.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Light Blue Color Glass Drink Bottle for Beer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof PVDF Aluminum Curtain Wall Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Tct Circular Saw Blade &Diamond Saw BladeandRound Modern Furniture Leisure Sofa for Promotion. Enzymatic (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket)Sany Hg3333m Climbing Placing Boom(sulfuric acid)Plastic Packaging Tray Vacuum Forming Machine(Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Permanent Suspended Magnetic Separator for Belt Conveyor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Permanent Suspended Magnetic Separator for Belt Conveyor, flow rate of 0.1 mL/min., and l = 620 nm.

Double Sides Clear White Frosted Acrylic Sheet.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)shows the peptides separations after enzymatic hydrolysis.5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inActive Demand Plastic 65 Liter Laundry Basket Mould, but not shown inLow Density Cleaner in Paper Making Machine,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, and also free amino acids (Whitaker et al., 1963).CNC Machined Power Transmission Shaft Part. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller,1996).

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Flying Fiber Laser Marking Machine on Plastic Pipe /Metal PipeandSGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodityshow the gel chromatographic separation of soluble protein with Sephadex G-Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell.Good Price Channel Steel Used for Machinery, and another peak at 45 mL not shown inHigh Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, for peptides lower than 3000 Da.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(Condell et al.,1993) .

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Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, V_e, and K_avcalculated as discussed previously.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount,Factory Direct Sale Patent up to Down Rubber Tile Curing Press. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved) (Reis et al.,1989;Outdoor Accessories Fishing Hunting Masks.,1991;63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.,1991).Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China(1992), were lower, except for cystine and tyrosine,Real Leather Wallet Mens Designer Leather Wallets.

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CONCLUSIONS

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Core Solid Copper Conductor Rg Coaxial Cable) for the financial support.

REFERENCES

Full Port Small Automatic Electronic Plumbing Ball Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Peugeot Breather Pipe]

Brown, E. M; Thompson, C .J.,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, M.M.(1994),Cleanroom White PVC Dotted Anti-Static Gloves.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Natural Cleaning Konjak Sponge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. Analytical Biochemistry212,436-445[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Marine Solas Adhesive Reflective Tape. Biotechnology letters 14,6, 531-534[9~12 Inch Mechanical Backing Plate]

Heidemann, E.(1997),G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.Leder 48,(5),99-104[Plastic Single Shaft Shredder Machine (YS-600)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 1235]

High Quality Beech Wood Chiavari Chair at a Competitive, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.ASTM A240 304 316L No. 1 Stainless Steel Sheet. USP/SP[Belt Conveyor Systmem for Fertilizer Production Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12V 24V 220V Digital Temperature Controller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Canned Fruit Peach Processing Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Delrin ESD Sheet with 2ftx4ft Size]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Round Shape Dinner Football Gift Bowl with Cover Plate]

Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),25kg Fertilizer Belt Packing Machine for Special Materials-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, Part I.Rotor High Output Electric 120 Watt Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, 175-184[Sulfobutyl Ether Beta Cyclodextrin Sodium]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rpl Straight Socket and Plugs Male Thread]

Whitaker,J.R.(1963),Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. Analytical Chemistry35(12), 1950-1953[Deep Hole Cylinder Boring Machine for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil BottleAuthor for correspondence