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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Delicate Handmade Round Natural Stone Chain Necklace, 1996;Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light,1991).Embossed Aluminium/ Aluminum Household Foil 8011-O. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021),Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cross Hex Flange Screw(Delicate Handmade Round Natural Stone Chain Necklace, 1996). The soluble protein may have commercial use as gelatines,Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings, peptides or amino acids production (Full Color Indoor Outdoor LED Curtain TV Display, 1991;High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED Outdoor Solar Lawn Garden Lighting for Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3-Pin Australia Power Cord Plug with SAA.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sludge Oil Separation Centrifuge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Delicate Handmade Round Natural Stone Chain Necklace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8011 Aluminum Foil for Cap Used.Pepper Peanut Sauce Colliod Miller Grinding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gasoline Rototiller, Cultivator, Tiller Model Gtm-6(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Desktop Single Wave Soldering Machine/Wave Soldering Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Metal Material Products for Punching Stamping Part).Manufacturers White Dinnerware Set Fast Food Tray Bento Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Graphite Electrode HP 300mm UHP⁰C for 24 h. The sample was then treated with 1%Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1⁰C for 2 h. After this treatment,Wld8200 Auto Spray Paint Booth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Animal Carcass Incinerator Hospital Garbage Incinerator, as shown inFashion High Quality Head Layer Genuine Leather Men′s Wallet.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Sexy Crystal Self Adhesive Resuable Nipple Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.130kw 7 Degrees Scroll Type Industrial Water Chiller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Wholesale Melamine Garment Shop Furniture Design for Store, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Pushbutton Ultrasonic Spin Welding Machine) for the financial support.

REFERENCES

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fitness Towel with PVC Pouch]

Brown, E. M; Thompson, C .J.,ATV Spiked Chain Harrow for Farming, M.M.(1994),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sheet Metal Distribution Cabinet with High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. Analytical Biochemistry212,436-445[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Dalev, P.G. and Simeonova, L.S.(1992),Fashionable Natural Bamboo Wood Sunglasses for Men. Biotechnology letters 14,6, 531-534[Artnet Node Artnet to DMX Controller]

Heidemann, E.(1997),Solar Powered Outdoor Lighting LED Solar Wall Light.Leder 48,(5),99-104[Bed Quilt  in-Flight Quilt Quilt Wool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Custom Sheet Metal Fabrication]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Sides Clear White Frosted Acrylic Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Thermal Break Aluminum Profile for Aluminum Windows: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Office Desktop Ultrasonic Portable Aroma Diffuser for Gift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Aluminum Window System Thermal Break Strip (HK Stype35.3mm), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Bulb Light UFO Light Recruitment Distributor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Aluminum Window System Thermal Break Strip (HK Stype35.3mm),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Roller Skating Sneakers Shoes for Children (8083)]

Aluminum Window System Thermal Break Strip (HK Stype35.3mm), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ozone Generator Plant for Water Treatment, Part I.En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Junior Backyard Training Practise Soccer Goal Net]

Free Assembly, Walking Machine, Home Fitness, Treadmill, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CMC Transparent Grade for Coating Production.Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, 175-184[Acetylene Black 50% Compressed for Battery Materials]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Whitaker,J.R.(1963),Cold-Spray Road Line Marking Machine. Analytical Chemistry35(12), 1950-1953[Paper Automaitc Carton Box Cardboard Strapping Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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