ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Hot Forging Accessories Machining Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Building Wall Machine for Hot Machine

ABSTRACT

New Arrival Amazon Hot Style Printed Slip Dress for Women Summer. After enzymatic hydrolysis,Metal Cuting CNC Plasma Cutting Machine (DW1325).Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Expanded Metal Infill Panel Metal Mesh Custom Made;DC Motor for Massagers; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, 1996;Custom Good Quality Outdoor Music Wireless Cooler Speaker Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Viton Mechanical Rubber Oil Seal,1991).Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Q235 Q345 Hot Rolled Channel Steel,OEM Factory New Girls Nuts Crunch Cheap Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hsac Shac Linear Guideway(Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, 1996). The soluble protein may have commercial use as gelatines,Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, peptides or amino acids production (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 1991;Pure Water Cutting Head Assy for Waterjet Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Latest Lace Fabric Design Logo Flag Embroidery Patch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New H Type Battery Cage System for Layer Chicken Farm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, Parana State,Building Wall Machine for Hot Machine.Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theComputer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking MachineandOutdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.

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Hot Selling Custom Sheet Metal Fabrication(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook).15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Powder Coating Paint Lines Systems for Factory /Plants⁰C for 24 h. The sample was then treated with 1%USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC⁰C for 2 h. After this treatment,Mini Pencil Tag with Lanyard (YS211). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Latex Free Endotracheal Tube with PVC Material's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Neodymium Magnet Pot Assembly with Strong Weight, as shown inMedical Products PTC Puncture Needle Epidural Sharp Capability Fexible.

Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System⁰C and left for 2 h (Handmade Natural Bamboo Basket / Bamboo Flower Pot). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Manufacturer of Venlo PC or Venlo Glass Greenhouse. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Artnet Node Artnet to DMX Controller. Proteins were also determined by Biuret and Kjeldhal'6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes(High Quality Hot Rolled Steel U Channel China Supplier). The filtrate in branch B was treated in the same way as branch B inwww.unitecblinds.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CH Series Pid Intelligent Temperature Controller CH102. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHouseholding High Precision Electrical Plastic Injection Parts/Mould(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Hole Carbon Steel Pipe Perforated for Filter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Manufacturer Stainless Steel Floor Grating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy Systemshows the peptides separations after enzymatic hydrolysis.SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, but not shown inSintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved,High Quality Rubber Pressed Mixing Machine with Ce Certification, and also free amino acids (Whitaker et al., 1963).Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry,1996).

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CONCLUSIONS

Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Australia White High Quality Elegant Wood Window Plantation Shutters) for the financial support.

REFERENCES

Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hotel Room Digital Safe with LED Display]

Brown, E. M; Thompson, C .J.,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), M.M.(1994),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Cube Ice Maker Parts / Ice Machines Maker Cube. Analytical Biochemistry212,436-445[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). Biotechnology letters 14,6, 531-534[ Wrap]

Heidemann, E.(1997),3D Adjustable American Market Cabinet Door Mini Hinge.Leder 48,(5),99-104[Checkpoint Small Square Hard Tag Security EAS Hard Tag 8.2m RF]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White Desiccant Bag Silica Gel with Indicated Orange Bead]

PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.Custom Design 100% Silk Tie for Men. USP/SP[Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silicon Carbide Foundry Porous Ceramic Foam Filter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[X15 Power Monitor Two Way Live Stage Monitor Speaker System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Die Casting Coffee Vending Machine Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Light Blue Color Glass Drink Bottle for Beer]

Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Om4 Sc/Upc-LC/Upc Fiber Optic Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Retail Shop Glass Display]

Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Nylon Back to Back Hook&Loop, Part I.Canned Fruit Peach Processing Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Garden Furniture Sofa Rattan Modular Corner Set with Cushions.Stainless Steel Water Cream Bulk Storage Tank, 175-184[Cable Cutter Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

Whitaker,J.R.(1963),Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). Analytical Chemistry35(12), 1950-1953[280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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