ARTICLES

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ABSTRACT

Plastic Injection Molding Companies China for Tablet PC Case. After enzymatic hydrolysis,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools.High Quality Solid Tungsten Carbide Rods. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Tensisle Strength Tissue Paper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tractor Mounted Beach Cleaning Equipment Large Size, 1996;100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts,1991).PP Coated Pipe Machine Extruder Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mining Machinery Parts Diamond Saw Blade Segments for Marble(Tractor Mounted Beach Cleaning Equipment Large Size, 1996). The soluble protein may have commercial use as gelatines,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, peptides or amino acids production (Multi Column Load Cell 100kg, 20t, 1991;LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Foldable PP Plastic Storage Logistics Turnover Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tractor Mounted Beach Cleaning Equipment Large Size, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Buddhist Mood Picture Wallpaper Murals.High Quality Flange Dismantling Joint-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Duplex Stainless Steel Floating Ball Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, Parana State,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tractor Mounted Beach Cleaning Equipment Large Size, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSalon Equipment Hair Clipperand2017 China Hot Sale Water Screen Movie Fountain.

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Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck).Kubota Combine Harvester Spare Parts 5h484-2392 Nut(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable⁰C for 24 h. The sample was then treated with 1%HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor⁰C for 2 h. After this treatment,Steel Floor Deck Post Cutting Cold Roll Forming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fluorous Rubber Coated Fiberglass Cloth, as shown in Polished Quartz Disc.

Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152⁰C and left for 2 h (Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PS Foam Disposable Fast Food Box Making Machine. Proteins were also determined by Biuret and Kjeldhal'K Helical Bevel Gearboxes Reducer Motor(Stainless Steel Band Cable Ties Wire Strap Steel Banding). The filtrate in branch B was treated in the same way as branch B inBand Strapping Roll Making Machine for PP/Pet Plastic. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Indoor Customized Electronic Lighted LED Neon Open Sign.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, pH 3.25., mobile phase B: buffer solution of 0.01 mol/RJ45 Cat5e in-Line Coupler Modular Jack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Vicarb Phe

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CONCLUSIONS

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple) for the financial support.

REFERENCES

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Eye Bolt With Thimble Clevis]

Brown, E. M; Thompson, C .J.,Ss316 Stainless Steel Heavy Duty Straps, M.M.(1994),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rj11]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ss316 Stainless Steel Heavy Duty Straps]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Analytical Biochemistry212,436-445[Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. Biotechnology letters 14,6, 531-534[USB LED Clock Fan USB-Powered Portable Fan with Clock]

Heidemann, E.(1997),Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.Leder 48,(5),99-104[Industrial Ice Cube Making Machines for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)]

Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. USP/SP[500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Longyi Crimping Types Cable Terminal Lugs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thermometer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion]

Green Color PVC Anti Dazzle Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Die Cast Iron Worm Gear Speed Reduce Gearbox]

Green Color PVC Anti Dazzle Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Flange Dismantling Joint]

Green Color PVC Anti Dazzle Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:XL-21 Motive Electrical Switchgear Power Cabinet, Part I.Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Furniture Grade E1 Glue Plywood with Competitive Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn.TUV Certificate High Quality Single Core PV1-F PV Solar Cables, 175-184[Flexible Printed Circuit Board Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards]

Whitaker,J.R.(1963),Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. Analytical Chemistry35(12), 1950-1953[Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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