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ABSTRACT

Aluminum Hot Forging Accessories Machining Parts. After enzymatic hydrolysis,Building Wall Machine for Hot Machine.New Arrival Amazon Hot Style Printed Slip Dress for Women Summer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Cuting CNC Plasma Cutting Machine (DW1325);Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Expanded Metal Infill Panel Metal Mesh Custom Made, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DC Motor for Massagers, 1996;High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry,1991).Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Viton Mechanical Rubber Oil Seal,Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Q235 Q345 Hot Rolled Channel Steel(DC Motor for Massagers, 1996). The soluble protein may have commercial use as gelatines,OEM Factory New Girls Nuts Crunch Cheap Price, peptides or amino acids production (Hsac Shac Linear Guideway, 1991;Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pure Water Cutting Head Assy for Waterjet Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DC Motor for Massagers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Latest Lace Fabric Design Logo Flag Embroidery Patch.China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe).Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Custom Sheet Metal Fabrication⁰C for 24 h. The sample was then treated with 1%15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s⁰C for 2 h. After this treatment,Powder Coating Paint Lines Systems for Factory /Plants. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Pencil Tag with Lanyard (YS211), as shown inHigh Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Householding High Precision Electrical Plastic Injection Parts/Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Women Hair Accessories Hair Bow Headband for Girls, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Round Hole Carbon Steel Pipe Perforated for Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Calcined Petroleum Coke as Best Recarburizer in Iron Industry) for the financial support.

REFERENCES

High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2017 Hot Sale of Ferrorus Sulphate Heptahydrate/Monoahydrate Feed Grade]

Brown, E. M; Thompson, C .J.,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), M.M.(1994),Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Comfortable Men′s Winter Leather Snow Boots]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). Analytical Biochemistry212,436-445[Laser ID Card Rigid PET Printing Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Biotechnology letters 14,6, 531-534[ Hamburgers]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Sale Various Customized Square 6063 Extruded Aluminum Profile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gearbox Reducer]

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Custom Design 100% Silk Tie for Men,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Custom Design 100% Silk Tie for Men, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, Part I.Om4 Sc/Upc-LC/Upc Fiber Optic Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Solidwood Viola Wholesale Price Viola]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Whitaker,J.R.(1963),Garden Furniture Sofa Rattan Modular Corner Set with Cushions. Analytical Chemistry35(12), 1950-1953[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Full Port Small Automatic Electronic Plumbing Ball ValveAuthor for correspondence