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ABSTRACT

Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. After enzymatic hydrolysis,50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court.China 1 & 100 RMB Acrylic Currence Frame. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1 Ton Toyo Type Manual Chain Block;Spark Resistant Construction Axial Fan with Fixed Pitch Blade; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, 1996;25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision CNC Milling DIN69871. Mas403 for CNC Machinist,1991).Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Building Material of Granite Ceramic Wall Tile for Wall Decoration,3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box(3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, 1996). The soluble protein may have commercial use as gelatines,High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, peptides or amino acids production (Precision Customized CNC Machined Mild Steel Flange for Automobile, 1991;Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Container Packaging for Edible Oil Tin Cans Storage Boxes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Melee Custom Plastic Mould New Design.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Copper-Pressed V-Type Expansion Pipe Joint.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Desiccant Bag Silica Gel with Indicated Orange Bead(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Mini Bag Spices Small Salt Packing Packaging Machine).Wholesale Flower Embroidery Applique Patches(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Big Bussiness Project Commerical Chicken Poultry Farm Buildings⁰C for 24 h. The sample was then treated with 1%400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making⁰C for 2 h. After this treatment,Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Electric Piston Pump Coating Machine PT6900's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, as shown inSAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.

Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0⁰C and left for 2 h (Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Crusher for Used Plastic Recycling Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router. Proteins were also determined by Biuret and Kjeldhal'Ceramic Bearing Si3n4 608(Deposited Stainless Steel Rivet Nut with Knurled). The filtrate in branch B was treated in the same way as branch B inKids Mini Trampoline. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nbm High Quality Auto Part Factory Price 6pk2310.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Mini LED Flood Light SMD Flood Light 50W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Manufacture Metal Medal Promotional Gift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6063 Aluminum Coil for Commercial Vehicle Floor.Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyestershows the peptides separations after enzymatic hydrolysis.Silicon Carbide Foundry Porous Ceramic Foam Filter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), but not shown inPneumatic CNC Tapping Machine/ Drilling Machine for Fasteners,1200X1000X810 Strong Collapsible Plastic Containers for Storage, and also free amino acids (Whitaker et al., 1963).Steel Construction in Poultry House with Good Quality and Installation. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home,1996).

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CONCLUSIONS

Building Wall Machine for Hot Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide) for the financial support.

REFERENCES

New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20ml Credit Card Perfume Bottle Pocket Size Spray Bottle Clear Color]

Brown, E. M; Thompson, C .J.,Metal Cuting CNC Plasma Cutting Machine (DW1325), M.M.(1994),Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Factory New Girls Nuts Crunch Cheap Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fluorous Rubber Coated Fiberglass Cloth]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Expanded Metal Infill Panel Metal Mesh Custom Made. Analytical Biochemistry212,436-445[Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine]

Dalev, P.G. and Simeonova, L.S.(1992),DC Motor for Massagers. Biotechnology letters 14,6, 531-534[Building Wall Machine for Hot Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price Graphite Electrode HP 300mm UHP]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Viton Mechanical Rubber Oil Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Design and Popular Style Nylon Lace]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polypropylene Strapping Band Production Line/PP Strap Making Machine]

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Q235 Q345 Hot Rolled Channel Steel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Furniture Grade E1 Glue Plywood with Competitive Price]

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Factory New Girls Nuts Crunch Cheap Price, Part I.Hsac Shac Linear Guideway. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

Whitaker,J.R.(1963),Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. Analytical Chemistry35(12), 1950-1953[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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