ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Biodegradable Disposible Straw Pulp Food Box Ppp-1007. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card

ABSTRACT

Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. After enzymatic hydrolysis,NBR Latex Adhesive for Glove.Cheap Wooden House Zinc Plated Angle Bracket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag, 1996;Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet,1991).12 Inch King Mattress Latex Foam Memory Foam Mattress. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt(Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag, 1996). The soluble protein may have commercial use as gelatines,Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable, peptides or amino acids production (Longyi Crimping Types Cable Terminal Lugs, 1991;Rigid PVC Foam Board for FRP Marine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Furniture Gold PVD Plasma Coating Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment.Steel Pipe Rust Removal Shot Blasting Cleaning Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hydraulic Press Corrugated Cold Roll Automatically Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Paper New Year Greeting Cards Wedding Invitations Cards).High Quality Solid Tungsten Carbide Rods(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover⁰C for 24 h. The sample was then treated with 1%Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved⁰C for 2 h. After this treatment,High Tensisle Strength Tissue Paper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tractor Mounted Beach Cleaning Equipment Large Size's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, as shown inElectric Heating Industrial Jacketed Glass Reactor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulti Column Load Cell 100kg, 20t(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Foldable PP Plastic Storage Logistics Turnover Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder) for the financial support.

REFERENCES

PS Foam Disposable Fast Food Box Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Three Phase 50/60 Hz Pv Inverter]

Brown, E. M; Thompson, C .J.,K Helical Bevel Gearboxes Reducer Motor, M.M.(1994),Indoor Customized Electronic Lighted LED Neon Open Sign.The Journal of the American Leather Chemists Association (JALCA)89(7)[Light Blue Color Glass Drink Bottle for Beer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Analytical Biochemistry212,436-445[ Retail Shop Glass Display]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Biotechnology letters 14,6, 531-534[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Heidemann, E.(1997),Custom Free Logo Design Cheap Hand Flag (NF01F02021).Leder 48,(5),99-104[Diesel Generator Parts AVR Ki-Davr-95s for Kipor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-RJ45 Cat5e in-Line Coupler Modular Jack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.pointsourcingservices.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry]

Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[KERONG Security Safe Combination Smart Key Card Cabinet Door Lock]

Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, Part I.High Quality Disposable Blood Transfusion Set with Filter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194).High Quality Brushless DC Electric Motor for Industrial Deduster, 175-184[Double Side Flex PCB Board with High Quality Better Price Manufacturer FPC Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Power Consumption Silicone Sex Doll Vending Machine]

Whitaker,J.R.(1963),China Factory Light Soda Ash /Sodium Carbonate. Analytical Chemistry35(12), 1950-1953[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination PartsAuthor for correspondence