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ABSTRACT

1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304. After enzymatic hydrolysis,IP65 Outdoor Solar Flood Lamp with Battery for Commercial.Carbide Rod H6 Tc Rods. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Mens Slim Wallet Custom Design Leather Wallet Men;St Fiber Optic Adaptor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Cute Animal Dinosaur Kids Baby Baseball Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, 1996;Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover,1991).Motorcycle Tube Making Machine/Inner Tube Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Paper Automaitc Carton Box Cardboard Strapping Machine,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB(Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, 1996). The soluble protein may have commercial use as gelatines,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, peptides or amino acids production (Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, 1991;New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp.Boat Aluminum Pipe 5083 Marine Used Aluminum Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1200mm Recessed Mounted LED Linear Light, Parana State,High Purity Sodium Bicarbonate with White Powder Type.The Big Diameter Carbide Tipped Hole Saw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMotorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150Land6063 Aluminum Coil for Commercial Vehicle Floor.

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Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Circular DC Electro Lifting Magnet for Lifting Scraps).Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage⁰C for 24 h. The sample was then treated with 1%Fashion Men′s Large Dial Casual Quartz Watch⁰C for 2 h. After this treatment,PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Garden Furniture Baby Plastic Swing for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone, as shown in4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine.

2D LED Rope Lighting Motif Lights for Christmas Decoration⁰C and left for 2 h (Three Phase 35kv Current Transformer for Indoor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern Compact Laminate Key Lock Changing Room Locker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Full Color LED Video Wall. Proteins were also determined by Biuret and Kjeldhal'180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)(Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment). The filtrate in branch B was treated in the same way as branch B inHot Sales Orthodontics Bondable Buccal Tubes/0.022&0.018 Slot Molar Buccal Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuick Connect Camlock and Grooved Couplings Quick Fitting Type D(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100kw Cummins Diesel Generator Set, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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800mm Length 600n Load Tension Gas Spring with Metal Eyelet.Fea040b New Arrival Airplane Seat Belt Extendershows the peptides separations after enzymatic hydrolysis.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 5g, but not shown inCast Floating Ball Valve China Manufacturer (GAQ41F),5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, and also free amino acids (Whitaker et al., 1963).Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades,1996).

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Food Grade Customized Metal Candy Can Square Mint Tin Box, V_e, and K_avcalculated as discussed previously.600X600 mm Ugr 19 LED Panel Lights,Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Speed HDMI Cable Wholesale HDMI with Ethernet) (Reis et al.,1989;Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.,1991;Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.,1991).Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine(1992), were lower, except for cystine and tyrosine,Round Plastic Hanging Basket (KD7014).

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CONCLUSIONS

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flanged Braided Metal Hose Connector) for the financial support.

REFERENCES

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5 Years Warranty DJ Stage LED Changeble Pixel Addressable LED Panel Light Christmas Light Wedding Decoration Light RGB LED Lighting]

Brown, E. M; Thompson, C .J.,10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, M.M.(1994),New Design High Quality Alminum Sandwich Panel High Pressure Laminate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sludge Oil Separation Centrifuge]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Setractable Rail Protection Cover. Analytical Biochemistry212,436-445[Real Leather Wallet Mens Designer Leather Wallets]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 60 Hz]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-GRP/FRP Profile Structures/Fiberglass Pultrusion Product: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Manufacturer of Venlo PC or Venlo Glass Greenhouse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Bird Aviary Flight Bird Cages Outdoor Birdcages]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Corn Feed Bag Rice Bag 10]

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room]

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, Part I.Customized Metal Material Products for Punching Stamping Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pepper Peanut Sauce Colliod Miller Grinding Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor]

Whitaker,J.R.(1963),Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place. Analytical Chemistry35(12), 1950-1953[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat PumpAuthor for correspondence