ARTICLES

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ABSTRACT

Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw. After enzymatic hydrolysis,Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag.Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, 1996;Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable,1991).Longyi Crimping Types Cable Terminal Lugs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rigid PVC Foam Board for FRP Marine,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)(HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Furniture Gold PVD Plasma Coating Machine, peptides or amino acids production (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1991;Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Bread Crumb Processing Line and Making Extruder Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder.China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Tractor Mounted Beach Cleaning Equipment Large Size(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump).100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts⁰C for 24 h. The sample was then treated with 1%PP Coated Pipe Machine Extruder Machine⁰C for 2 h. After this treatment,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mining Machinery Parts Diamond Saw Blade Segments for Marble, as shown inC Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel.

Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface⁰C and left for 2 h (Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi Column Load Cell 100kg, 20t. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. Proteins were also determined by Biuret and Kjeldhal'Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck(4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head). The filtrate in branch B was treated in the same way as branch B inCheap Ceramic Bowls From China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Foldable PP Plastic Storage Logistics Turnover Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuddhist Mood Picture Wallpaper Murals(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Duplex Stainless Steel Floating Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Kubota Combine Harvester Spare Parts 5h484-2392 Nut.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Partsshows the peptides separations after enzymatic hydrolysis.Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRound Plastic Hanging Basket (KD7014), but not shown inK Helical Bevel Gearboxes Reducer Motor,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, and also free amino acids (Whitaker et al., 1963).Steel Floor Deck Post Cutting Cold Roll Forming Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance,1996).

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280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), V_e, and K_avcalculated as discussed previously.PS Foam Disposable Fast Food Box Making Machine,K Helical Bevel Gearboxes Reducer Motor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality 304 Stainless Steel Sheet for Home Decoration Material) (Reis et al.,1989;Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment.,1991;Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt.,1991).Indoor Customized Electronic Lighted LED Neon Open Sign(1992), were lower, except for cystine and tyrosine,Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar.

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CONCLUSIONS

Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Solid Tungsten Carbide Rods) for the financial support.

REFERENCES

Custom Free Logo Design Cheap Hand Flag (NF01F02021), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Magnetic Snap Button]

Brown, E. M; Thompson, C .J.,125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, M.M.(1994),Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X.The Journal of the American Leather Chemists Association (JALCA)89(7)[Femo Ferro Molybdenum Powder Lump 55/60]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-RJ45 Cat5e in-Line Coupler Modular Jack. Analytical Biochemistry212,436-445[Automotive Rubber Parts for Any Rubber Material]

Dalev, P.G. and Simeonova, L.S.(1992),Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. Biotechnology letters 14,6, 531-534[3-Pin Australia Power Cord Plug with SAA]

Heidemann, E.(1997),Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper.Leder 48,(5),99-104[Sex Toys For Her]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Partition Paper Slotter Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fire Alarm Detector 24VDC Reflective Beam Detector]

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Brushless DC Electric Motor for Industrial Deduster-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20]

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Light Soda Ash /Sodium Carbonate, Part I.Regular Size Cigarette Rolling Papers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

304L Stainless Steel U Channel Good Performance, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Junior Backyard Training Practise Soccer Goal Net, 175-184[Roto Molded Ice Box Cooler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Whitaker,J.R.(1963),350mm Diamond Blade for Granite. Analytical Chemistry35(12), 1950-1953[Design Luxury Moon Cake Box with Hot Stamp Logo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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