ARTICLES

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ABSTRACT

Rigid PVC Foam Board for FRP Marine. After enzymatic hydrolysis,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Furniture Gold PVD Plasma Coating Machine;Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Pipe Rust Removal Shot Blasting Cleaning Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1996;2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Bread Crumb Processing Line and Making Extruder Machine,1991).Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Injection Molding Companies China for Tablet PC Case(Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1996). The soluble protein may have commercial use as gelatines,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, peptides or amino acids production (High Quality Solid Tungsten Carbide Rods, 1991;Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Tensisle Strength Tissue Paper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tractor Mounted Beach Cleaning Equipment Large Size. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap.Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Coated Pipe Machine Extruder Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Multi Column Load Cell 100kg, 20t, Parana State,Longyi Crimping Types Cable Terminal Lugs.LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Iron Wire Mesh Chain Link Mesh Security FenceandHigh Quality Brushless DC Electric Motor for Industrial Deduster.

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Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses).Foldable PP Plastic Storage Logistics Turnover Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005⁰C for 24 h. The sample was then treated with 1%Buddhist Mood Picture Wallpaper Murals⁰C for 2 h. After this treatment,High Quality Flange Dismantling Joint. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Duplex Stainless Steel Floating Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, as shown inRoller Hearth Heating Furnace for Gas Cylindrer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKubota Combine Harvester Spare Parts 5h484-2392 Nut(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel Floor Deck Post Cutting Cold Roll Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

New Mason Cup Frosted Drink Bottle Juice Glass Mason JarandFluorous Rubber Coated Fiberglass Cloth. Enzymatic (Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152)280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)(sulfuric acid)PS Foam Disposable Fast Food Box Making Machine(Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%K Helical Bevel Gearboxes Reducer Motor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%K Helical Bevel Gearboxes Reducer Motor, flow rate of 0.1 mL/min., and l = 620 nm.

Indoor Customized Electronic Lighted LED Neon Open Sign.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Carshows the peptides separations after enzymatic hydrolysis.Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, but not shown inFree Shipping Wholesale Peruvian Human Hair Weave,Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, and also free amino acids (Whitaker et al., 1963).Custom Free Logo Design Cheap Hand Flag (NF01F02021). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine,1996).

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CONCLUSIONS

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mining Machinery Parts Diamond Saw Blade Segments for Marble) for the financial support.

REFERENCES

High Quality Brushless DC Electric Motor for Industrial Deduster, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Control System Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,China Factory Light Soda Ash /Sodium Carbonate, M.M.(1994),Regular Size Cigarette Rolling Papers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tefude Feeding and Packaging Line for Food with Tray]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304L Stainless Steel U Channel Good Performance. Analytical Biochemistry212,436-445[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Dalev, P.G. and Simeonova, L.S.(1992),Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). Biotechnology letters 14,6, 531-534[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

Heidemann, E.(1997),Junior Backyard Training Practise Soccer Goal Net.Leder 48,(5),99-104[Ozone Aging Tester]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.uzo-pak.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

Laser ID Card Rigid PET Printing Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Arrival Plush Animal Ride for Kids for Shopping Center-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

Laser ID Card Rigid PET Printing Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Laser ID Card Rigid PET Printing Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, Part I.(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

Ss316 Stainless Steel Heavy Duty Straps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus.High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, 175-184[Fitness]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Whitaker,J.R.(1963),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. Analytical Chemistry35(12), 1950-1953[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose ToolsAuthor for correspondence