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ABSTRACT

Outdoor Full Color LED Video Wall. After enzymatic hydrolysis,180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125).Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality White Calcined Alumina Powder for Ceramic Industry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, 1996;100kw Cummins Diesel Generator Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle,1991).Luxury Hotel Furniture Antique Design Marble Side Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator,Commercial Double Lane Inflatable Carnival Bungee Run Game,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair(500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, 1996). The soluble protein may have commercial use as gelatines,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, peptides or amino acids production (800mm Length 600n Load Tension Gas Spring with Metal Eyelet, 1991;Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.100 Liter Cooking Pots for Jam-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Customized Metal Candy Can Square Mint Tin Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man).New Design High Quality Alminum Sandwich Panel High Pressure Laminate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Setractable Rail Protection Cover⁰C for 24 h. The sample was then treated with 1%Natural Birch F&B Veneer Furniture Plywood⁰C for 2 h. After this treatment,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Tire Repair Tool Kit, Tire Repair Strip's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Performance 915MHz PCB Antenna, as shown inHigh Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.

GRP/FRP Profile Structures/Fiberglass Pultrusion Product⁰C and left for 2 h (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. Proteins were also determined by Biuret and Kjeldhal'Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light(2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment). The filtrate in branch B was treated in the same way as branch B inFolding Bamboo Dish Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Metal Material Products for Punching Stamping Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Theme Park Inflatable Bouncer for Sale (BJ-B16), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Muliti-Size Light Hot Stamping Foil for PS Frame, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LED Bulb Light UFO Light Recruitment DistributorandWaterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Enzymatic (50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court)China 1 & 100 RMB Acrylic Currence Frame(sulfuric acid)1 Ton Toyo Type Manual Chain Block(500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Spark Resistant Construction Axial Fan with Fixed Pitch Blade, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Spark Resistant Construction Axial Fan with Fixed Pitch Blade, flow rate of 0.1 mL/min., and l = 620 nm.

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl.High Quality Women′s Suit in Reasonable Priceshows the peptides separations after enzymatic hydrolysis.3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHsac Shac Linear Guideway, but not shown inFRP Pultruded Pipe or GRP Profile,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), and also free amino acids (Whitaker et al., 1963).Precision CNC Milling DIN69871. Mas403 for CNC Machinist. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine,1996).

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Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, V_e, and K_avcalculated as discussed previously.High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop,Precision Customized CNC Machined Mild Steel Flange for Automobile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Mini LED Flood Light SMD Flood Light 50W) (Reis et al.,1989;800mm Length 600n Load Tension Gas Spring with Metal Eyelet.,1991;100kw Cummins Diesel Generator Set.,1991).Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing(1992), were lower, except for cystine and tyrosine,Metal Container Packaging for Edible Oil Tin Cans Storage Boxes.

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CONCLUSIONS

Melee Custom Plastic Mould New Design. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine) for the financial support.

REFERENCES

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[25mva Transformer 25000kVA 33kv 35kv Power Transformer]

Brown, E. M; Thompson, C .J.,Copper-Pressed V-Type Expansion Pipe Joint, M.M.(1994),Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Tensisle Strength Tissue Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Desiccant Bag Silica Gel with Indicated Orange Bead. Analytical Biochemistry212,436-445[High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Iron Wire Mesh Chain Link Mesh Security Fence. Biotechnology letters 14,6, 531-534[Big Bussiness Project Commerical Chicken Poultry Farm Buildings]

Heidemann, E.(1997),TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump.Leder 48,(5),99-104[Multi Bag Filter Housing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dental Phantom Head]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fully Automatic Water Bottle Lids Labeling Machine]

360 Degree Maverick Angler Fishing Kayak with Rotating Seat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody]

360 Degree Maverick Angler Fishing Kayak with Rotating Seat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Screw/Countersunk Head Screw 6 Ribs with Wing]

360 Degree Maverick Angler Fishing Kayak with Rotating Seat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Flower Embroidery Applique Patches, Part I.Big Bussiness Project Commerical Chicken Poultry Farm Buildings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HPMC Hydroxy Propyl Methyl Cellulose Additives]

400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20.New Electric Piston Pump Coating Machine PT6900, 175-184[Custom Sizes Paper Cardboard Sliding Gift Packing Drawer Boxes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology]

Whitaker,J.R.(1963),200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. Analytical Chemistry35(12), 1950-1953[Paper Egg Box Forming Molding Production Line/ Egg Tray Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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